Project description:The Chinese sturgeon (Acipenser sinensis) is anadromous fish distributed in Yangtze River and East China Sea. In this study, we reported cleft-palate Chinese sturgeons in artificial population for the first time. In order to explore the genetic basis of palate malformation in A. sinensis, Illumina RNA-seq technology was used to analyze the transcriptome data of normal and cleft-palate individuals in farmed Chinese sturgeons. Raw reads were obtained and assembled into 808,612 unigenes, with an average length of 509.33 bp and an N50 of 574 bp. Sequence similarity analyses against four public databases (Nr, Uniprot, KEGG and COGs) found 158,642 unigenes that can be annotated. GABAergic synapse and TGF-β signal pathway were the most two enriched pathways with high Richfactor in the analyses of different expressed genes. In these two signal pathways, six genes (GABRA4, GS, GNS, S6K, PITX2, and BMP8) were found as cleft-palate genes in Chinese sturgeon. These findings contribute to our understanding of the genetic basis of cleft palate in sturgeon, while simultaneously adding to our knowledge about craniofacial development.
Project description:Transcriptome profiling reveals cleft palate-related genes in Chinese sturgeon (Acipenser sinensis)
| PRJNA445484 | ENA
Project description:Pathogenic infection and immune-related gene expression of Chinese sturgeon (Acipenser Sinensis) challenged by Citrobacter freundii
Project description:Agarwood is a kind of precious traditional Chinese medicine, the scarity of natural agarwood limits its wide application and resulted in the high price of agarwood. Agarwood formed when Aquilaria sinensis was suffered from chemical stimulus, mechanical damage and fungal infection, however, the molecular mechanism of which has not been fully elucidated. In this study, the quantitative proteomic analysis using iTRAQ technology combined with transcriptome analysis on chemically induced A. sinensis were firstly performed to elucidate the agarwood formation mechanism by formic acid stimulus. 3,029 proteins were detected, 504 differential proteins that showed at least 2-fold differences in their expression levels were selected ,
Project description:Sturgeon species, considered living fossils, exhibit unique reproductive characteristics, making it crucial to comprehend the molecular processes governing the formation and quality of their eggs. However, there is a notable lack of comprehensive data concerning the protein composition analysis of sturgeon eggs and ovarian fluid (OF) and their functional significance. To address this knowledge gap, this study aimed to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon eggs and OF using liquid chromatography-mass spectrometry (LC-MS/MS). A total of 566 proteins were identified in eggs, while 617 proteins were identified in OF, with 772 proteins showing differential abundance. Among the differentially abundant proteins, 407 were more abundant in eggs, while 365 showed higher abundance in OF. Furthermore, we identified 288 proteins specific to eggs and 339 proteins specific to OF, along with the top ten most abundant proteins in each. Functional annotation analysis unveiled enriched metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, as well as protein ubiquitination and translation, in eggs. Conversely, ovarian fluid proteins primarily associated with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of actin cytoskeleton. This study presents the first comprehensive characterization of the protein composition of sturgeon ovarian fluid and eggs, shedding light on their distinct functional roles. The findings not only advance our understanding of sturgeon reproduction but also shed light on egg-OF signaling and origin of the OF proteins. Moreover the identified proteins offer potential biomarkers for predicting egg quality contributing to the development of effective breeding strategies for sturgeon species.
Project description:Nutrition is an important part of the protection process of Yangze sturgeon. This study tested the transcriptome levels of brain, liver and spleen after feeding different fat source diets to Yangze sturgeon.