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Simultaneous measurement of DNA repair activities and mRNA abundance in single cells

ABSTRACT: Simultaneous measurement of DNA repair activities and mRNA abundance in single cells

PROVIDER: PRJNA534019 | ENA |

REPOSITORIES: ENA

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Simultaneous measurement of DNA repair activities and mRNA abundance in single cells

Project description:New methods that measure the static abundance of DNA, RNA, and proteins in single cells are rapidly transforming biology. But these methods do not provide insight into dynamic cellular activities, precluding a complete understanding of cellular heterogeneity. We developed a method to measure enzymatic activities in single cells and used it to simultaneously measure DNA repair enzyme activities and mRNA expression. By including polyadenylated DNA hairpin substrates with defined lesions in a single-cell mRNA sequencing experiment, we measured repair activities by capturing repair intermediates and products catalyzed by activities in single cells.

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A targeted multi-omic analysis approach measures protein expression and low abundance transcripts on the single cell level

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