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Next Generation Sequencing to identify the gene expression profile of cardiosphere-derived cells (CDCs) and c-kitpos cardiac progenitor (c-kitpos CPC) cells derived from adult and young donors

ABSTRACT: Next Generation Sequencing to identify the gene expression profile of cardiosphere-derived cells (CDCs) and c-kitpos cardiac progenitor (c-kitpos CPC) cells derived from adult and young donors

PROVIDER: PRJNA534229 | ENA |

REPOSITORIES: ENA

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Extracellular vesicles (EV) from IFNγ-primed cardiosphere-derived cells (CDCs)

Project description:Characterization of proteins in extracellular vesicles (EV) from cardiosphere-derived cells (CDCs) of a clinically relevant pig model. Additionally, considering that priming stem cells with inflammatory stimuli may increase the therapeutic potential of released vesicles, here we studied the dynamic changes that take place in the EV from IFNγ-primed CDCs.

2020-05-15 | PXD016434 | Pride

Next Generation Sequencing to identify the gene expression profile of cardiosphere-derived cells (CDCs) and c-kitpos cardiac progenitor (c-kitpos CPC) cells derived from adult and young donors

Project description:In this study we therefore focused on 2 heart-derived progenitor cell populations that have been recently introduced in clinical translation, autologous c-kitpos–selected CPCs and CDCs. For the first time we compared molecular signatures and proliferation and differentiation characteristics of progenitor cells from young donor hearts (c-kitpos yCPC and yCDC) with cells obtained from adult donors with advanced ischemic disease (c-kitpos aCPC and aCDC). Here we report that culture expanded CDCs derived from adult donors have a distinct transcriptional profile with greater cell cycle activity and superior in vitro paracrine growth factor release compared to yCDCs. The molecular signature of aduIt CDCs favours cardiac repair via pro-angiogenic, cytotrophic and immunomodulatory effects while cardiac transdifferentiation potential is modest and comparable in all progenitor cell populations.

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comparison of primary epicardium explant derived CDCs and primary Epicardial cells

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2022-02-18 | GSE189275 | GEO

Transcriptome analysis of mdx hearts and skeletal muscles treated with cardiac progenitor cells and their exosomes

Project description:Aged mdx mice were treated with 2.5x105 cardiosphere-derived cells (CDCs) or 2.0x109 exosomes intravenously. Hearts and skeletal muscles were harvested 3 weeks post-treatment and prepared for RNA sequencing.

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Single Cell RNA Sequencing of Cultured Primary Tissue Cells.

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