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Cohesin has a deleterious effect on heterochromatin assembly in fission yeast cells lacking Pds5

ABSTRACT: Cohesin has a deleterious effect on heterochromatin assembly in fission yeast cells lacking Pds5

PROVIDER: PRJNA534333 | ENA |

REPOSITORIES: ENA

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Cohesin has a deleterious effect on heterochromatin assembly in fission yeast cells lacking Pds5

Project description:Occupancy profiling of lysine 9 dimethylated histone H3 in fission yeast. Occupancy profiling of Pds5 proteins in fission yeast.

2020-04-22 | GSE130233 | GEO

Cohesin Impedes Heterochromatin Assembly in Fission Yeast Cells Lacking Pds5.

Project description: Not available

| S-EPMC6727797 | biostudies-literature

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [ChIP-seq]

Project description:Cohesin regulates sister chromatids cohesion but also contributes to chromosome folding by promoting the formation of chromatin loops, a process proposed to be mediated by loop extrusion. PDS5 plays a crucial role in regulating the cohesin dynamic on chromatin, mainly through WAPL-mediated release activity and the opponent ESCO1/2-sororin dependent pathway. However, the exact function of PDS5 on cohesin-mediated chromatin looping remains ambiguous. Two versions of PDS5 exist in vertebrates, PDS5A and PDS5B. Here, we construct cells for rapid PDS5A or PDS5B degradation in PLC/PRF/5 cell line with the FKBP12F36V-dTAG degron system to perform loss of function studies. Combining Hi-C, ChIP-seq for cohesin regulators, and RNA-seq data, we describe the redundant and discrete roles of PDS5A and PDS5B in three-dimensional genome organization and gene expression.

2022-10-08 | GSE209845 | GEO

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [RNA-seq]

2022-10-08 | GSE209848 | GEO

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [Hi-C]

2022-10-08 | GSE209846 | GEO

Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL and PDS5 proteins

Project description:Mammalian genomes are organized into compartments, topologically-associating domains (TADs) and loops to facilitate gene regulation and other chromosomal functions. Compartments are formed by nucleosomal interactions, but how TADs and loops are generated is unknown. It has been proposed that cohesin forms these structures by extruding loops until it encounters CTCF, but direct evidence for this hypothesis is missing. Here we show that cohesin suppresses compartments but is essential for TADs and loops, that CTCF defines their boundaries, and that WAPL and its PDS5 binding partners control the length of chromatin loops. In the absence of WAPL and PDS5 proteins, cohesin passes CTCF sites with increased frequency, forms extended chromatin loops, accumulates in axial chromosomal positions (vermicelli) and condenses chromosomes to an extent normally only seen in mitosis. These results show that cohesin has an essential genome-wide function in mediating long-range chromatin interactions and support the hypothesis that cohesin creates these by loop extrusion, until it is delayed by CTCF in a manner dependent on PDS5 proteins, or until it is released from DNA by WAPL.

2017-12-07 | GSE102884 | GEO

Pds5 promotes cohesin acetylation and stable cohesin-chromosome interaction.

Project description: Not available

| S-EPMC3388792 | biostudies-other

Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast

Project description:The meiotic cohesin Rec8 is required for the stepwise segregation of chromosomes during the two rounds of meiotic division. By directly measuring chromosome compaction in living cells of the fission yeast Schizosaccharomyces pombe, we found an additional role for the meiotic cohesin in the compaction of chromosomes during meiotic prophase. In the absence of Rec8, chromosomes were decompacted relative to those of wild-type cells. Conversely, loss of the cohesin-associated protein Pds5 resulted in hyper-compaction. While this hyper-compaction requires Rec8, binding of Rec8 to chromatin was reduced in the absence of Pds5, indicating that Pds5 promotes chromosome association of Rec8. To explain these observations, we propose that meiotic prophase chromosomes are organized as chromatin loops emanating from a Rec8-containing axis; the absence of Rec8 disrupts the axis, resulting in disorganized chromosomes, whereas reduced Rec8 loading results in a longitudinally compacted axis with fewer attachment points and longer chromatin loops. Keywords: ChIP-chip analysis

2006-08-23 | GSE5284 | GEO

Roles of the Brca2 and Wapl complexes with Pds5 in sister chromatid cohesion, cohesin localization, and gene expression [ChIP-seq]

Project description:The Pds5, Wapl and Brca2 proteins were mapped by ChIP-seq, and the effects of their depletion on the binding of cohesin subunits and the Nipped-B cohesin loader were examined by ChIP-seq

2018-02-05 | GSE109384 | GEO

Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast

2010-07-01 | E-GEOD-5284 | biostudies-arrayexpress

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