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Transcriptomic analysis of Arabidopsis wild-type (Col-0), phyb-9, jaz10 and jaz10phyB-9 plants to methyl jasmonate (MeJA)

ABSTRACT: Transcriptomic analysis of Arabidopsis wild-type (Col-0), phyb-9, jaz10 and jaz10phyB-9 plants to methyl jasmonate (MeJA)

PROVIDER: PRJNA534468 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of Arabidopsis wild-type (Col-0), phyb-9, jaz10 and jaz10phyB-9 plants to methyl jasmonate (MeJA)

Project description:We used Affymetrix 1.0 ST arrays to compare the transcriptome of 4 weeks‐day‐old plants sprayed with 250uM of MeJA or mock (control). For the experiments we used wild-type (Col-0), phyB-9 and jaz10 single mutants and jaz10phyb double mutant. Samples were harvested after 3hours of induction with MeJA or mock. We performed a first analysis with the transcriptomes of wild‐type (Col-0) to identify those group of genes that are up‐ and down‐regulated by MeJA treatment (2 fold-changes). We founded 719 genes whose expression was statistically significantly different between the two treatments. To gain knowledge into the regulation of phyB over these set of 719 genes, we contrasted their expression levels against phyB transcriptome. By cluster analysis, we identified five major groups of genes, where in two of them the transcript levels increased in presence of MeJA (clusters 1 and 2) and three whose expression decrease after MeJA treatment (Cluster 3, 4 and 5).

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Detection of Methyl jasmonate (MeJA) in Plant root VOCs

Project description:Methyl jasmonate (MeJA) is a well-known plant hormone known for plant defense and plant-plant signaling. However, most of the studies are focussed on its aboveground presence and functions. Here we report that MeJA is also released by plant roots in a volatile form. More importantly, it is shown in Arabidopsis growing in natural conditions in soil.

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Effect of methyl jasmonate treatment on rice leaves of wild-type and rice COI mutatns

Project description:Analysis of leaves of wild-type and rice COI mutants treated with methyl jasmonate (MeJA). Results provide the role of rice COI on response to jasmonic acid.

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