Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

ABSTRACT: Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

PROVIDER: PRJNA534481 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8950060.fastq.gz	Fastqsanger.gz
	SRR8950061.fastq.gz	Fastqsanger.gz
	SRR8950062.fastq.gz	Fastqsanger.gz
	SRR8950063.fastq.gz	Fastqsanger.gz
	SRR8950064.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

Project description:We performed RNA-seq to identify transcriptional profiles of itch-activated CeA neurons. To do this, we crossed FosTRAP mice to the Cre-dependent tdTomato flox-stop reporter line as described above. We then injected chloroquine into the nape of the neck, paired with injection of 4-OHT to FosTRAP expression of tdTomato in itch-activated neurons. The CeA was harvested, the cells dissociated and sorted by FACS, and tdTomato+ cells were compared to TdTomato- cells.

2020-04-23 | GSE130268 | GEO

Central Amygdala TRAP-Seq of Drd2 neurons

Project description:Central Amygdala TRAP-Seq of Drd2 neurons

| PRJNA472614 | ENA

Molecular profiling of insular cortex neurons projecting to the central amygdala

Project description:We used the retro-TRAP protocol (Ekstrand et al 2014) to profile insular cortex neurons that project to the central amygdala

2020-12-24 | GSE163743 | GEO

Nppb Neurons Are Sensors Of Mast Cell-Induced Itch

Project description:Itch is an unpleasant skin sensation which can be triggered by exposure to many chemicals including those released by mast cells. The Nppb-expressing class of sensory neurons when activated elicit scratching responses in mice, however, it is unclear which itch-inducing agents stimulate these cells and the receptors involved. Here, we identify receptors expressed by Nppb-neurons and demonstrate the functional importance of these receptors as sensors of endogenous pruritogens released by mast cells. Our search for receptors in Nppb-neurons revealed that they express leukotriene, serotonin, and sphingosine-1-phosphate receptors. Targeted cell ablation, calcium imaging of primary sensory neurons, and conditional receptor knockout studies demonstrate that these receptors induce itch by the direct stimulation of Nppb-neurons and neurotransmission through the canonical GRP-dependent spinal cord itch pathway. Together our results define a molecular and cellular pathway for mast cell-induced itch.

2019-03-26 | GSE125626 | GEO

Transcriptomic landscape of the central amygdala of the mouse

Project description:Inhibitory neurons are the predominant type of neuron in the mouse central amygdala. Here, we examined diversity in this cell type using single-cell RNA-seq.

2022-10-19 | GSE184666 | GEO

Molecular profiling of insular cortex neurons projecting to the central amygdala

Project description:Molecular profiling of insular cortex neurons projecting to the central amygdala

| PRJNA687416 | ENA

A central extended amygdala circuit that modulates anxiety

Project description:Both the amygdala and the bed nucleus of the stria terminalis (BNST) have been implicated in maladaptive anxiety characteristic of anxiety disorders. However, the underlying circuit and cellular mechanisms have remained elusive. Here we show that mice with Erbb4 gene deficiency in somatostatin-expressing (SOM+) neurons exhibit heightened anxiety as measured in the elevated plus maze test and the open field test, two assays commonly used to assess anxiety-related behaviors in rodents. Using a combination of electrophysiological, molecular, genetic and pharmacological techniques we demonstrate that the abnormal anxiety in the mutant mice is caused by enhanced excitatory synaptic inputs onto SOM+ neurons in the central amygdala (CeA), and the resulting reduction in inhibition onto downstream SOM+ neurons in the BNST. Notably, our results indicate that an increase in dynorphin signaling in SOM+ CeA neurons mediates the paradoxical reduction in inhibition onto SOM+ BNST neurons, and that the consequent enhanced activity of SOM+ BNST neurons is both necessary for and sufficient to drive the elevated anxiety. Finally, we show that the elevated anxiety and the associated synaptic dysfunctions and increased dynorphin signaling in the CeA-BNST circuit of the Erbb4 mutant mice can be recapitulated by stress in wild-type mice. Together, our results unravel previously unknown circuit and cellular processes in the central extended amygdala that can cause maladaptive anxiety.

2018-05-31 | GSE113979 | GEO

Single cell RNA-sequencing in the mouse central amygdala (CEA)

Project description:The central amygdala (CEA) is a brain region that consists of primarily GABAergic neurons. It has been widely investigated for its role in many innate and adaptive behaviors, such as appetitive and defensive behaviors. Despite the complex functions of CEA, the molecular diversity of CEA neurons has not been systemically examined. Here, we performed single-cell RNA-sequencing (scRNA-Seq) in the CEA to classify the molecularly defined neuron types in this region.

2023-02-03 | GSE213828 | GEO

Gene expression of central amygdala of mice

Project description:Purpose: The goals of this study are to screen out the changes of gene expression profile in response to high fat diet, an mRNA-seq was performed in central amygdala Methods: 6-week-old male wild-type mice were fed with high fat diet (HFD, containing 45% fat) or normal chow diet (ND) for 5 months. And then their central amygdala tissues were removed and total RNA was extracted to perform RNA sequencing. Results:The KEGG pathway analysis showed that genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after HFD Conclusions: Our study represents the first detailed analysis of central amygdalar transcriptomes of mice subjected to high fat diet, with biologic replicates, generated by RNA-seq technology. Our results show genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after high fat diet feeding,which hint at the importance of ion channel in central amygdala after high fat feeding.

2022-02-17 | GSE196719 | GEO

Gene expression of central amygdala of mice

Project description:Gene expression of central amygdala of mice

| PRJNA806964 | ENA