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Investigating Proteome and Transcriptome Defense Response of Grapes Induced by Yarrowia lipolytica

ABSTRACT: Investigating Proteome and Transcriptome Defense Response of Grapes Induced by Yarrowia lipolytica

PROVIDER: PRJNA534483 | ENA |

REPOSITORIES: ENA

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Investigating Proteome and Transcriptome Defense Response of Grapes Induced by Yarrowia lipolytica

Project description:We report the application of next generation sequencing technology for analyzing the differentially expressed genes of grapes after treated with or without Y. lipolytica. After analyzing the differentially expressed gene, we selected some important genes related to the resistance of grapes to do RT-qPCR.

2019-04-26 | GSE130267 | GEO

Investigating Proteome and Transcriptome Defense Response of Pears Induced by Wickerhamomyces anomalus

Project description:Investigating Proteome and Transcriptome Defense Response of Pears Induced by Wickerhamomyces anomalus

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Absolute expression level of Yarrowia lipolytica genes

Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Yarrowia lipolytica genes Total RNA was collected in mid-log phase from Yarrowia lipolytica cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Yarrowia lipolytica.

2010-07-06 | E-GEOD-22192 | biostudies-arrayexpress

Expression analysis of Yarrowia lipolytica Y4184 snf1 mutant

Project description:Investigation of whole genome gene expression level changes in a Yarrowia lipolytica Y4184 snf1 mutant, compared to the Y4184U+. The Y4184 is an engineered strain to produce eicosapentaenoic acid (EPA) via expression of a M-NM-^T9 elongase/M-NM-^T8 desaturase pathway, and is derived from Yarrowia lipolytica ATCC#20362. A six chip study using total RNA recovered from three separate cultures of Yarrowia lipolytica Y4184Ura+ strains and three separate cultures of Y4184 snf1 mutant strains in which YlSNF1 gene is deleted entirely. Each chip measures the expression level of 6708 genes from Yarrowia lipolytica ATCC#20362 with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

2013-09-25 | E-GEOD-45611 | biostudies-arrayexpress

Expression analysis of Yarrowia lipolytica Y4184 snf1 mutant

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Transcriptome change in response to ochratoxin A (OTA) in Yarrowia lipolytica Y-2

Project description:The yeast Yarrowia lipolytica Y-2 shown the capability to degrade OTA by the intracellular enzymes. However, the enzymes which risponsible for the degradation process was unkonown. Transcriptome change in response to mycotoxin OTA was analyzed. The molecular mechanism of Yarrowia lipolytica Y-2 withstand OTA was revealed.

2018-11-29 | GSE123081 | GEO

Absolute expression level of Yarrowia lipolytica genes

Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Yarrowia lipolytica genes

2010-07-06 | GSE22192 | GEO

Comparative transcriptomics of Yarrowia lipolytica on different substrates

Project description:batch culture of Yarrowia lipolytica strain W29

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Transcriptome of Yarrowia lipolytica strain E150 delta-Ku70 on glucose

Project description:batch culture of Yarrowia lipolytica strain E150 delta-Ku70

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Yarrowia lipolytica biolipid production during D-stat setup. [Part 2]

Project description:Second part of the time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, obtained during a controlled decellerostat (D-stat) setup. A nitrogen limitation was applied during the course of the D-stat to initiate and control de novo biolipid synthesis. Yarrowia lipolytica Agilent microarray, one-color staining. Each time sample was spotted in triplicate (except for sample 26, for which a technical replicate of replicate r1 was performed).

2015-01-01 | E-GEOD-35446 | biostudies-arrayexpress

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