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Hematopoietic stem cells in perisinusoidal niches are protected from ageing [aged, GFP-label retaining HSCs]

ABSTRACT: Hematopoietic stem cells in perisinusoidal niches are protected from ageing [aged, GFP-label retaining HSCs]

PROVIDER: PRJNA535431 | ENA |

REPOSITORIES: ENA

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Hematopoietic stem cells in perisinusoidal niches are protected from ageing [aged, GFP-label retaining HSCs]

Project description:With ageing, intrinsic hematopoietic stem cell (HSC) activity decreases, resulting in impaired tissue homeostasis, reduced engraftment following transplantation and increased susceptibility to diseases. However, whether ageing affects also the HSC niche impairing the capacity to support HSC function is still largely unknown. Here, by using in-vivo long-term label retention assays we demonstrate that aged labelling retaining (LR) HSCs, which are in the old mice the most quiescent HSC subpopulation with the highest regenerative capacity and cellular polarity, reside predominantly in perisinusoidal niches. Furthermore, we demonstrate that sinusoidal niches and perisinusoidal Nes-GFPlow cells are uniquely preserved in shape, morphology and number upon ageing. Finally, we show that myeloablative chemotherapy can selectively disrupt aged sinusoidal niches, which is linked to hematopoietic failure and decreased survival of aged mice. Overall, our data characterize for the first time the functional alterations of the aged HSC niche and unveil that perisinusoidal niches are uniquely preserved and protect HSCs from ageing.

2019-09-02 | GSE130298 | GEO

HMEC Label-retaining cells

Project description:Gene expression after mammosphere culture, label-retaining cells

| EGAD00010001968 | EGA

Hematopoietic stem cells in perisinusoidal niches are protected from ageing [young and aged BM CD45-CD31+ endothelial cells]

2019-09-02 | GSE129726 | GEO

HMEC Non-label-retaining cells

Project description:Gene expression after mammosphere culture, non-label-retaining cells

| EGAD00010001966 | EGA

Murine Kidney Papilla Cells: Control GFP- vs. GFP+ label retaining cells. Cell populations were separated by FACS.

Project description:Transcriptional profiling of mouse kidney papilla cells comparing control GFP- cells with GFP+ labeled cells. Mouse kidney papillae were dissected. GFP- and GFP+ cell populations were isolated by FACS. The latter represent slow cycling label retaining cells (LRCs). Two-condition experiment, GFP- vs. GFP+ label retaining cells cells. Biological replicates: 5 control GFP- replicates, 5 GFP+ label retaining cell replicates.

2015-08-05 | E-GEOD-71693 | biostudies-arrayexpress

Hematopoietic stem cells in perisinusoidal niches are protected from ageing

Project description:Hematopoietic stem cells in perisinusoidal niches are protected from ageing

| PRJNA535426 | ENA

Expression profile of sweat gland label retaining cells (LRCs)

Project description:We have used the slow cycling property, found in hair follicle stem cells, to look for LRCs in sweat glands as putative stem cells. Gene expression profiling allowed us to determine the common and unique genes expressed in SG LRCs and non-LRCs. This allowed us to probe for the signaling pathways active in this skin appendage as well as determine potential molecular markers of SG LRCs. Sweat gland label retaining cells (LRCs) and surrounding basal layer cells were isolated from K5TetOffTreH2BGFP mice after a 4 week chase with doxycycline and compared to the basal layer of the sole's epidermis in 2 independent experiments.

2013-12-24 | E-GEOD-49011 | biostudies-arrayexpress

Murine Kidney Papilla Cells: Control GFP- vs. GFP+ label retaining cells. Cell populations were separated by FACS.

2015-08-05 | GSE71693 | GEO

A label-retaining but unipotent cell population resides in biliary compartment of mammalian liver.

Project description:Cells with slow proliferation kinetics that retain the nuclear label over long time periods – the label-retaining cells (LRCs) – represent multipotent stem cells in a number of adult tissues. Since the identity of liver LRCs (LLRCs) had remained elusive we utilized a genetic approach to reveal LLRCs in normal non-injured livers and characterized their regenerative properties in vivo and in culture. We found that LLRCs were located in biliary vessels and participated in the regeneration of biliary but not hepatocyte injury. In culture experiments the sorted LLRCs displayed an enhanced self-renewal capacity but a unipotent biliary differentiation potential. Transcriptome analysis revealed a unique set of tumorigenesis- and nervous system-related genes upregulated in LLRCs when compared to non-LRC cholangiocytes. We conclude that the LLRCs established during the normal morphogenesis of the liver do not represent a multipotent primitive somatic stem cell population but act as unipotent biliary progenitor cells.

2017-03-01 | GSE90160 | GEO

Intestinal label-retaining cells are secretory precursors expressing Lgr5

Project description:The epithelial lining of the small intestine is continuously renewed from a small number of stem cells including Lgr5 expressing crypt base cells that have been shown to be a rapidly cycling stem cell population in homeostasis. Alternative markers of intestinal stem cells have variously identified populations as rapidly cycling or quiescent with proposed roles for the latter as a parallel or reserve stem cell population. However, the exact nature of quiescent crypt cells remains unknown. Here by applying novel mouse models that permit their isolation and characterisation as label-retaining cells (LRCs), and for the first time performing lineage tracing from them, we show quiescent cells to be committed secretory precursors that are capable of recall to the stem cell state. We reveal LRCs to be a small subset of the rapidly cycling Lgr5 expressing population committed along the Paneth-enteroendocrine lineage. Significantly, after injury and subsequent regeneration they are clonogenic, as shown by both lineage tracing and organoid growth demonstrating that they can be recalled to the stem cell pool. These findings in establishing quiescent cells as an effective clonogenic reserve during injury provides a motivation for investigating their role in the maintenance of cancer growth following adjuvant treatment.

2013-02-27 | GSE43772 | GEO

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