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DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [PacBio]

ABSTRACT: DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [PacBio]

PROVIDER: PRJNA540244 | ENA |

REPOSITORIES: ENA

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DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [PacBio]

Project description:We used Pacific Biosciences Single Molecule Real-Time sequencing platform to identify modified motifs in an RM deficient strain of Streptococcus pyogenes

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DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes

Project description:DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes

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DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [RNA-seq]

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DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [RNA-seq]

Project description:We used Illumina NGS to measure mRNA levels and perform subsequent differential expression analysis between wild type and RM system deficient strains of Streptococcus pyogenes

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RNA-seq (Illumina and PacBio) of hESC

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Project description:Genetically programmed deaths play important roles in the biology of unicellular prokaryotic cells. Some gene complexes force their maintenance on the host bacterial cells by killing cells that have lost them. This form of programmed death called post-segregational killing or genetic addiction is brought about by several Type II restriction-modification gene complexes, through restriction attack on the undermethylated chromosome, and underlie their behavior as selfish mobile elements. To learn the genetic steps to death, we examined how carriage and loss of PaeR7I restriction-modification gene complex affect host Escherichia coli cells through transcriptome and experimental analyses. The PaeR7I complex was on a temperature-sensitive plasmid so that the killing was induced by a temperature shift. We used microarrays to detail the global program of gene expression underlying cell death process mediated by PaeR7I restriction-modification system in E. coli. Experiment Overall Design: Post-segregational cell killing was induced by blocking replication of a temperature sensitive plasmid carrying PaeR7I RM gene complex by shifting up the cultivation temperature. At 30℃, the permissive temperature for the plasmid replication, growth as monitored by OD660, of MG1655/pTN9 (r+m+) was indistinguishable from those of MG1655/pTN11 (r-m+) and MG1655/pHSG415 (vector). Cell death was observed at least 4 h after the temperature shift only in MG1655/pTN9 (r+m+) as in the previous works; the increase in viable cell counts stopped and resulted in decrease of cell viability. To analyze global gene expression when cells went to death, we performed transcriptome analysis 0 h, 1h, 1 h 50 min, and 3h after the temperature shift. We used Affimetrix E. coli antisense genome array. Experiments were performed independently twice.

2009-07-04 | E-GEOD-7326 | biostudies-arrayexpress

Neisseria gonorrhoeae with knock-out of NgoAXP (ngo0545::km) and NgoAV (ngo406::cm) Restriction-modification system versus wild type

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Genome-wide H3K4me3 and gene expression before and after methionine restriction in human cancer cells and mouse liver

Project description:In this study, we compared genomic H3K4me3 and gene expression profiles before and after methionine restriction in human cancer cells and mouse liver. The goals are to understand the influences of nutrient availability on genomic architecture of histone modification and gene expression.

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