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Gene expression profiling identifies potential biomarkers for colorectal cancer using NanoString nCounter assay

ABSTRACT: Gene expression profiling identifies potential biomarkers for colorectal cancer using NanoString nCounter assay

PROVIDER: PRJNA540604 | ENA |

REPOSITORIES: ENA

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Gene expression profiling identifies potential biomarkers for colorectal cancer using NanoString nCounter assay

Project description:The purpose of this study was to identify the differentially expressed genes and new potential predictive and prognostic molecular biomarkers for CRC. We identified 101 genes that were upregulated and 76 genes that were downregulated in metastatic tumors. After validation, a reduced set of 27 genes revealed evident correlation with CRC in the tumor tissue. Several genes in the pan-cancer panel showed significant differential expression, which were more universal than others in the CRC tissue. Since some of them have not been reported as being directly related to CRC yet, future mechanism studies can be designed based on this study.Our study demonstrated NanoString nCounter assay could serve as an alternative approach for gene expression analysis and identified several unreported differently expressed genes in CRC patients, which may provide some important clues for more in-depth study of CRC and may serve as potential predictive molecular biomarkers for clinical diagnosis application as well.

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Concordance of PAM50 molecular subtyping between oligonucleotide microarray and NanoString nCounter assay for Taiwanese breast cancer

Project description:Five molecular subtypes have been identified according to intrinsic genes transcription. Microarrays have been adopted for molecular subtyping in addition to the original NanoString nCounter assay. This study aimed to evaluate subtyping consistency among Taiwanese breast cancers. Our study showed that fundamental discrepancy exists between distinct gene expression measuring approaches, and cross platform equivalence should not be overemphasized with too much extrapolation.

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miRQC data for NanoString nCounter miRNA Expression Assays.

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nCounter analysis of mRNAs associated with GBM tissue using a Nanostring custom assay

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Nanostring nCounter expression analysis of sea lamprey embryos and adult testes (Petromyzon marinus)

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