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Next Generation Sequencing Analysis of Beta-actin knockout (KO) and wildtype (WT) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Under Adipogenic Induced Conditions

ABSTRACT: Next Generation Sequencing Analysis of Beta-actin knockout (KO) and wildtype (WT) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Under Adipogenic Induced Conditions

PROVIDER: PRJNA541301 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Analysis of Beta-actin knockout (KO) and wildtype (WT) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Under Adipogenic Induced Conditions

Project description:mRNA profiles of beta-actin knockout (KO) and wild-type (WT) MEFs induced to adipocyte-like feature to study how global transcriptome changes during differentiation

2019-05-07 | GSE130765 | GEO

Next Generation Sequencing Analysis of chemically reprogrammed osteoblasts from Beta-actin Wildtype (WT) Heterozygotes (HET) and knockout (KO) Mouse Embryonic Fibroblast (MEFs)

Project description:mRNA profiles of chemically reprogrammed osteoblasts from Beta-actin Wildtype (WT) Heterozygotes (HET) and knockout (KO) Mouse Embryonic Fibroblast (MEFs)

2019-08-06 | GSE135392 | GEO

RNA-Seq analysis of beta-actin knockout MEFs expressing NLS-tagged beta-actin

Project description:RNA-Seq analysis of beta-actin knockout MEFs expressing NLS-tagged beta-actin to study role of actin in transcription

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Next Generation Sequencing Analysis of Beta-actin knockout (KO) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Stimulated by Viral Mimics

Project description:mRNA profiles of beta-actin knockout (KO) MEFs stimulated by viral mimics to study how global transcriptome changes after innate viral immunity activation

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ChIP-Seq of H3K27ac in Actb-/- MEFs expressing NLS-tagged beta-actin

Project description:Genome-wide ChIP-Seq of H3K27ac in β-actin KO MEFs expressing NLS-tagged beta-actin to assess the role of nulcear actin in regulating histone acetylation

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RNAseq from Mfn1/2 knockout MEFs with adipogenic differentiation

Project description:Objective: A biallelic missense mutation in mitofusin 2 (MFN2) causes multiple symmetric lipomatosis and partial lipodystrophy, implicating disruption of mitochondrial fusion or interaction with other organelles in adipocyte differentiation, growth and/or survival. In this study, we aimed to document the impact of loss of mitofusin 1 (Mfn1) or 2 (Mfn2) on adipogenesis in cultured cells. Methods: We characterised adipocyte differentiation of wildtype (WT), Mfn1-/- and Mfn2-/- mouse embryonic fibroblasts (MEFs) and 3T3-L1 preadipocytes in which Mfn1 or 2 levels were reduced using siRNA. Results: Mfn1-/- MEFs displayed striking fragmentation of the mitochondrial network, with surprisingly enhanced propensity to differentiate into adipocytes, as assessed by lipid accumulation, expression of adipocyte markers (Plin1, Fabp4, Glut4, Adipoq), and insulin-stimulated glucose uptake. RNA sequencing revealed a corresponding pro-adipogenic transcriptional profile including Pparg upregulation. Mfn2-/- MEFs also had a disrupted mitochondrial morphology, but in contrast to Mfn1-/- MEFs they showed reduced expression of adipocyte markers. Mfn1 and Mfn2 siRNA mediated knockdown studies in 3T3-L1 adipocytes generally replicated these findings. Conclusions: Loss of Mfn1 but not Mfn2 in cultured pre-adipocyte models is pro-adipogenic. This suggests distinct, non-redundant roles for the two mitofusin orthologues in adipocyte differentiation.

2023-06-01 | GSE232502 | GEO

Role of beta-actin in mouse embryonic fibroblasts (MEFs)

Project description:Role of beta-actin in mouse embryonic fibroblasts (MEFs)

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Next Generation Sequencing Analysis of Beta-actin knockout (KO) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Stimulated by Viral Mimics

Project description:Next Generation Sequencing Analysis of Beta-actin knockout (KO) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Stimulated by Viral Mimics

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Mouse Embryonic Fibroblasts (MEFs) Adipogenic Potential: A Comprehensive Transcriptome Analysis

Project description:Mouse Embryonic Fibroblasts (MEFs) Adipogenic Potential: A Comprehensive Transcriptome Analysis

| PRJNA640338 | ENA

Next Generation Sequencing Analysis of chemically reprogrammed osteoblasts from Beta-actin Wildtype (WT) Heterozygotes (HET) and knockout (KO) Mouse Embryonic Fibroblast (MEFs)

Project description:Next Generation Sequencing Analysis of chemically reprogrammed osteoblasts from Beta-actin Wildtype (WT) Heterozygotes (HET) and knockout (KO) Mouse Embryonic Fibroblast (MEFs)

| PRJNA558732 | ENA

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