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Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge [in vitro]

ABSTRACT: Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge [in vitro]

PROVIDER: PRJNA541482 | ENA |

REPOSITORIES: ENA

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Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge

Project description:Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge

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Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge [in vivo]

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Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge [in vitro]

Project description:The communication between immune and central nervous system represents an emerging field in neuroscience with many potential consequences both in basic and translational medicine. Among the plethora of mechanisms which might be involved, it is now clear that that immune molecules released upon inflammatory state can modulate different physiological aspects of brain development, resulting in long-lasting pathological consequences which may interfere with normal behavior during adulthood. A common aspect of this immune molecule is to promote deep transcriptional changes responsible for long-term effects. Single-cell RNA sequencing is now becoming a cutting-edge technology for studying the genomic rearrangement occurring at single cell level in different experimental models, thus opening a new frontier in medicine. Here, by using primary cultured neurons as a simplified model for studying neuronal development, we study the long-term consequences of the proinflammatory cytokine interleukine-6 (IL-6) on developing neurons through a single-cell sequencing approach. Based on the immuneprofiling of the analyzed cells, we identified different neuronal and non-neuronal cell clusters whose transcriptional profile changed specifically upon the inflammatory stimulation.

2021-05-13 | GSE130803 | GEO

Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge [in vivo]

2021-07-20 | GSE180344 | GEO

Single cell transcriptional profiling of cultured developing neurons upon inflammatory challenge

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-07-20 | GSE180345 | GEO

Histone deacetylase inhibition enhances antimicrobial peptide but not inflammatory cytokine expression upon bacterial challenge

Project description:We report expression of genes from human Caco-2 cells (subclone TC7) after inhibition of histone deacetylases by trichostatin A, upon an E. coli bacterial challenge

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Poly(A) RNA profiling upon Gld2 knockdown in cultured hippocampal neurons

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Poly(A) RNA profiling upon Gld2 knockdown in cultured hippocampal neurons

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Single-cell RNA-sequencing overviews global inflammatory response in in vitro organ cultured testes

Project description:Background: Production of functional sperms from spermatogonial stem cells in vitro is important for understanding of biological mechanisms underlying physiological spermatogenesis and for treatment of male infertility. However, comparing with the in vivo testes, in vitro spermatogenesis is severely lower efficiency. Here we describe abnormalities occurring in early stage of in vitro spermatogenesis at single cell resolution. Results: While spermatogenesis was similarly progressed between in vivo and in vitro-cultured testes, noticeable acute inflammatory response occurred right after in vitro culture of neonatal testes not only in immune cells but also non-immune testicular somatic cells. Inhibitor treatment revealed NLRP3 inflammasome signaling is key to the inflammation occurring in in vitro cultured testes We also found accumulation of damaged/dead germ cells in in vitro cultured testes, which may be due to dysfunction of sertoli cell phagocytosis. Conclusion: Our data revealed tissue-wide sterile inflammation occurring in in vitro cultured testes, in which DAMPs - NLRP3 inflammasome axis may key element. Thus, these abnormal testicular microenvironments may be a cause of low spermatogenesis efficiency of in vitro spermatogenesis.

2022-12-31 | GSE189805 | GEO

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