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Homo sapiens isolate:hESC

ABSTRACT: Human Embryonic Stem Cell Line H9 and H7 for UTX Knockout by CRISPR/CAS9

PROVIDER: PRJNA542154 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9053010_1.fastq.gz	Fastqsanger.gz
	SRR9053010_2.fastq.gz	Fastqsanger.gz
	SRR9053011_1.fastq.gz	Fastqsanger.gz
	SRR9053011_2.fastq.gz	Fastqsanger.gz
	SRR9053012_1.fastq.gz	Fastqsanger.gz

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Homo sapiens isolate:hESC

Project description:Human Embryonic Stem Cell Line H9 for 53BP1 Knockout by CRISPR/CAS9

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RNA-seq of H9 hESC line carrying shRB expression cassette, and H9 hESC line carrying shRB expression cassette in addition to CRISPR/Cas9-mediated nonsense mutations on RBL1 and RBL2

Project description:In order to investigate the roles of retinoblastoma family genes during early human development, mutant H9 human embryonic stem cells were generated. Frameshift mutations were introduced in RBL1 and RBL2 genes using the CRISPR/Cas9 technology, and then the shRNA-expression cassette to knockdown RB upon tetracycline treatment was integrated. These cells were cultured in definitive endoderm differentiation conditions for 3 or 6 days with/without tetracycline.

2022-06-20 | E-MTAB-11109 | biostudies-arrayexpress

Haplotype-phased Callithrix jacchus embryonic stem cell line for genome editing using CRISPR/Cas9

Project description:Haplotype-phased Callithrix jacchus embryonic stem cell line for genome editing using CRISPR/Cas9

2018-07-09 | PRJEB27676 | EVA

Whole-genome sequencing of the CRISPR/Cas9-generated cell lines G12LS and C07 carrying a heterozygous and homozygous mutation in the RB1 gene and their parental human embryonic stem cell line H9.

Project description:Heterozygous and homozygous mutations were introduced to the human embryonic stem cell line H9 by using the CRISPR/Cas9-system. Since off-target effects can occur and high numbers of SNVs can be acquired during clonal selection, the generated cell lines and the parental cell line were analyzed by whole-genome sequencing.

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Short C-terminal Musashi-1 proteins regulate pluripotency states in embryonic stem cells

Project description:To understand the function of MSI1 in pluripotent stem cells, RNA-seq assays were performed on mouse embryonic stem cells R1, MSI1 knockout cell line R1-C5, human embryonic stem cells H9, RRM knockout cell line H9-C8, MSI1 full-length overexpression cell line H9-MSI1OE, MSI1C variant overexpression cell line H9-MSI1 (138-362) OE , H9-MSI1(272-362)OE. RNA bound by MSI1 in R1 and H9, and MSI1C variants MSI1 (138-362), MSI1(272-362) were detected using RIP-seq.

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RAMPAGE from H7-hESC (ENCSR022VBF)

Project description:The libraries contained in this experiment come from independent growths of embryonic stem cell line H7-hESC. They are stranded PE101 Illumina Hi-Seq RAMPAGE libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-11-08 | GSE87961 | GEO

total RNA-seq from H7-hESC (ENCSR490SQH)

Project description:The libraries contained in this experiment come from independent growths of uninduced embryonic stem cell line H7-hESC. They are stranded PE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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miRNA-seq of HEK293 cell lines on Illumina iSeq100 instrument

Project description:A validation experiment performed on HEK293 cell lines after genome editing. The design contains three duplicate runs consisted of: HEK293 wild type cell line HEK293 with MIR484 gene knockdown using CRISPR-Cas9 HEK293 with MIR185 gene knockout using CRISPR-Cas9

2023-06-16 | E-MTAB-13009 | biostudies-arrayexpress

RNA-seq of UGP2 mutant human embryonic stem cells and in vitro differentiated neural stem cells

Project description:We report transcriptome analysis of human embryonic stem cells and in vitro differentiated neural stem cells, comparing wild type H9, UGP2 homozygous knock-out and UGP2 mutant cells harboring a homozygous A>G nucleotide change affecting the start codon of UGP2 isoform 2 that was introduced by CRISPR-Cas9 engineering

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RNA-seq of wild-type and KLF17-null hESCs during chemical resetting from primed to naive pluripotency

Project description:Bulk RNA-seq of H9 human embryonic stem cells undergoing conversion from primed to naive pluripotency using the chemical/epigenetic resetting method in tt2iL+Go-based media conditions. The dataset includes three wild-type clones (WT1-3) and two KLF17-null clones (KO1-2) generated through CRISPR-Cas9-mediated gene editing. Samples were collected at day 0 (primed cells in mTeSR1 (StemCell Technologies)), then throughout resetting at day 2 (cells in cRM-1), day 8 (cells in cRM-2+XAV939, immediately prior to the first passage), naive passage 5 (p5), 7 (p7) and 10 (p10) (cells in tt2iL+Go).

2021-09-30 | E-MTAB-10914 | biostudies-arrayexpress

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