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Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

ABSTRACT: Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

PROVIDER: PRJNA542590 | ENA |

REPOSITORIES: ENA

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Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

Project description:Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. Here, we study the adverse effects of GO and amino-functionalized GO (GONH2) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Seq data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. Our work establishes the enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as biomedical nanomaterial.

2022-05-10 | GSE131070 | GEO

Single-cell mass cytometry reveals the impact of graphene nanomaterials with human primary immune cells

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A transcriptomic overview of lung and liver changes one day after pulmonary exposure to graphene and graphene oxide

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Project description:Phosphorylation of protein plays a key role in the regulation of many biological processes. Herein, a simple approach is developed by immobilization of Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO) as adsorbent for selective isolation of intact phosphoproteins from real samples, such as MCF-7 cell lysate.

2018-07-20 | PXD010524 | iProX

Methylation arrays (MethylationEPIC) of human lung epithelial BEAS-2B cells exposed to reduced graphene oxide

Project description:Nanomaterials have lots of promising applications, and concern has risen about their impact to human health. Here, we have analyzed the genome-wide DNA methylation changes associated to the exposure to reduced graphene oxide (rGO) in human lung epithelial cells. Six conditions were assayed, with two technical replicates per condition (12 arrays in total): control, 1 and 10 µg/mL of rGO for 15 or 30 days of exposure.

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2020-04-22 | GSE149013 | GEO

RNAseq of primary human monocyte derived macrophages (MDM) and differentiated THP-1 macrophages exposed to graphene-related (nano)materials (GRMs)

Project description:Occupational and consumer exposure to GRMs will increase but their impact on human health is still largely unknown. We sought to perform a transcriptome comparison of the bioresponses of MDM and THP-1 macrophages exposed to 5 or 20 µg/ml graphene oxide (GO) or graphene nanoplatelets (GNP) for 6 and 24 h to capture early and more persistent acute responses. Cristalline silica (DQ) was included as immunotoxic reference material.

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