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Variovorax enforce stereotypic root development

ABSTRACT: Variovorax enforce stereotypic root development

PROVIDER: PRJNA542788 | ENA |

REPOSITORIES: ENA

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Variovorax enforce stereotypic root development

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-05-15 | GSE131158 | GEO

RNA-seq of Arabidopsis thaliana seedlings growing axenically, or in the presence of different bacterial Synthetic Community treatments that induced different root morphology

Project description:This study evaluated the transcriptomic profiles of Arabidopsis thaliana (Col-0) plants grown along four SynCom treatments that induced differential primary root growth. Treatments Dropout Variovorax and DropoutVariovoraxBurkholderia induced primary root growth inhibition (RGI), while treatments Full and DropoutBurkholderia mantained a stereotypical long primary root.

2019-05-15 | GSE131156 | GEO

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action of microgravity on root development-Action of microgravity on root development

2012-07-31 | E-GEOD-39631 | biostudies-arrayexpress

An Nfic-Hedgehog Signaling Cascade Regulates Tooth Root Development

Project description:This study aimed to perform transcriptome profiling of Nfic-/- and corresponding control tooth germ at root initiation stage to identify differentially expressed for key regulators of root development. Coordination between the Hertwig’s Epithelial Root Sheath (HERS) and apical papilla (AP) is crucial for proper root development process. The Hedgehog (Hh) signaling pathway and Nfic are both involved in tooth root development.

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Variovorax

Project description:Variovorax sp. overview

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Root isoprene formation alters lateral root development Maja Miloradovic van Doorn

Project description:Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. All rights reserved.

2020-06-16 | PXD019789 | Pride

Opposing roles of E2A and Id3 that orchestrate and enforce the naive T cell fate

Project description:It is established that E2A and its antagonist, Id3, modulate developmental progression at the pre-TCR and TCR checkpoints. Here we show at a global scale how E2A promotes commitment to the T cell lineage and how pre-TCR mediated signalling affects E2A genome-wide occupancy. We find aberrant development of CD4 memory-like and TFH-like cells, T-B cell conjugates and, remarkably, B cell follicles in Id3-/-thymi. We also find that Id3-/-CD4 splenocytes exhibit increased numbers of TFH-like cells. We propose a model in which Id3 modulates the naive versus effector/memory cell fate. Collectively, these data show how E2A acts globally to orchestrate T-lineage development and that Id3 antagonizes E2A activity beyond the pre-TCR checkpoint to enforce the naive T cell fate. ChIP-Seq was performed in thymocytes isolated from either untreated Rag2-/-mice (DN3 cells) or Rag2-/- mice injected with anti-CD3e antibody (DN4 cells). ChIP used antibodies against either E2A or H3K4me1.

2011-07-31 | E-GEOD-30518 | biostudies-arrayexpress

Conserved Molecular Program for Root Development in Diverse Plants

Project description:We define genome-wide temporal expression patterns of root development genes in seven vascular plants, enabling a detailed comparative analysis of the molecular development of a single organ across diverse species. Measuring mRNA expression of the root development zones in 7 vascular plants. Each measurement includes 3 biological replicates.

2015-08-24 | E-GEOD-64665 | biostudies-arrayexpress

Variovorax paradoxus

Project description:Variovorax paradoxus overview

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