ABSTRACT: RNA-seq analysis reveals spatial and sex differences in pectoralis major muscle of broiler chickens contributing to difference in susceptibility to wooden breast disease
Project description:Wooden Breast (WB) is a novel myopathy affecting the pectoralis major muscle of modern broiler chickens. We selected pectoralis fillet samples, a normal(healthy) fillet and a WB fillet.
Project description:Detection of differentially expressed genes in broiler Pectoralis major muscle affected by White Striping – Wooden Breast myopathies
Project description:Broiler industry is facing an increasing prevalence of breast myopathies such as white striping (WS) and wooden breast (WB), whose precise etiology remains poorly understood. In order to progress in the understanding of the structural changes and the molecular pathways involved in these myopathies, and identify biomarkers, a transcriptomic analysis using an 8×60K Agilent chicken microarray was performed. The study used pectoralis major muscles from three groups: slow-growing animals (n=8), fast-growing animals visually free from defects (n=8) or severely affected by both WS and WB (n=8). In addition, a weighted correlation network analysis was performed to investigate the relationship between modules of co-expressed genes and histological traits.
Project description:We report the results of comparative transcriptional profiling in the pectoralis major muscle of a modern production broiler line (Ross 708) and a legacy broiler line inbred since the late 1940s (Illinois) focusing on metabolic differences and differences in myogenic growth regulators Differential gene expression analysis between Ross 708 and Illinois pectoralis major at two post-hatch time points (D6 and D21) that bracket critical inflection point in the allometric growth characteristics of the muscle
Project description:The aim of the present study was to investigated the difference of Nrf2-regulated genes in livers between normal and heat-stressed chickens. The CUT&Tag and high-throughput sequencing technologies were used in this experiment. Results showed that 13171838- 15417444 clean reads were obtained in this study. These data suggested that there were many Nrf2- regulated genes in the liver of heat-stressed chicken.
Project description:We report the results of comparative transcriptional profiling in the pectoralis major muscle of a modern production broiler line (Ross 708) and a legacy broiler line inbred since the late 1940s (Illinois) focusing on metabolic differences and differences in myogenic growth regulators
