Project description:To better undersand the effects of drought stress on wheat developing seeds, the transcription profile of early developing wheat seeds under control and drought stress conditions were comparatively analyzed by using the Affymetrix wheat geneChip. Drought stress is a major yield-limiting factor for wheat. Wheat yields are particularly sensitive to drought stress during reproductive development. Early seed development stage is an important determinant of seed size, one of the yield components. We specifically examined the impact of drought stress imposed during postzygotic early seed development in wheat. We imposed a short-term drought stress on plants with day-old seeds and observed that even a short-duration drought stress significantly reduced the size of developing seeds as well as mature seeds. Drought stress delayed the developmental transition from syncytial to cellularized stage of endosperm. Coincident with reduced seed size and delayed endosperm development, a subset of genes associated with cytoskeleton organization was misregulated in developing seeds under drought-stressed. Several genes linked to hormone pathways were also differentially regulated in response to drought stress in early seeds. Notably, drought stress strongly repressed the expression of wheat storage protein genes such as gliadins, glutenins and avenins as early as 3 days after pollination. Our results provide new insights on how some of the early seed developmental events are impacted by water stress, and the underlying molecular pathways that can possibly impact both grain size and quality in wheat.

2015-09-15 | GSE70443 | GEO

Meninges from mice with different microbiomes

Project description:RNA was extracted from the meninges of mice from either Specific pathogen free or Germ free facilities or from the offspring of mice reconstituted with different human microbiomes.

2020-11-13 | GSE135620 | GEO

Gene expression analysis in a Brazilian wheat cultivar under drought

Project description:The central part of Brazil, consisting mostly of the Cerrado Biome, is considered to be the new frontier for increasing Brazilian wheat production. However, rainfed wheat production in that area must cope with drought stress. In order to better understand the drought response, we analyzed the mRNA profiling under drought in roots and leaves of the cultivar MGS1 Aliança (a well-adapted cultivar to the Cerrado). We identified 4,422 candidate genes in roots and leaves.

2016-11-29 | GSE81833 | GEO

Environmental adaptation of root-associated microbiomes

Project description:Environmental adaptation of root-associated microbiomes

| PRJEB33850 | ENA

Environmental adaptation of root-associated microbiomes

Project description:Environmental adaptation of root-associated microbiomes

| PRJEB33853 | ENA

Giessen FACE rRNA

Project description:Giessen FACE rRNA Metagenome

| PRJNA656997 | ENA

Paeonia ostii root-associated microbiomes

Project description:Paeonia ostii root-associated microbiomes Raw sequence reads