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Gene expression analysis of THP-1 cells cultured with or without BCAA

ABSTRACT: Gene expression analysis of THP-1 cells cultured with or without BCAA

PROVIDER: PRJNA545054 | ENA |

REPOSITORIES: ENA

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Gene expression analysis of THP-1 cells cultured with or without BCAA

Project description:The branched-chain amino acid (BCAA) metabolism plays pleiotropic roles in homeostasis. Here we show that human acute leukemia-initiating cells (LICs), but not normal hematopoietic stem cells, are heavily addicted to the BCAA metabolism, irrespective of myeloid or lymphoid types. To clarify how BCAA metabolism affect the gene expression of human acute leukemia cells, we examined the gene expression alteration in human acute leukemia cell lines in control and BCAA-resrticted culture conditions.

2019-07-01 | GSE131813 | GEO

Gene expression analysis of Kasumi9 cells cultured with or without BCAA

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Gene expression analysis of Jurkat cells cultured with or without BCAA

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Differentiation of cultured THP-1 cells

Project description:Libraries constructed from cultured THP-1 cells receiving PMA treatment or not. Keywords: other

2004-11-19 | GSE1439 | GEO

Gene expression analysis of human CD34+ ALL cells cultured with or without gabapentin

Project description:To clarify the global gene expression alteration in primary CD34+ ALL cells after BCAA metabolism inhibition, CD34+ AML cells were treated with or without gabapentin in vitro. After 9-12 hours of culture with or without gabapentin, FACS-purified CD34+ ALL cells were subjected to transcriptome analysis.

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Gene expression analysis of human CD34+ AML cells cultured with or without gabapentin

Project description:To clarify the global gene expression alteration in primary CD34+ AML cells after BCAA metabolism inhibition, CD34+ AML cells were treated with or without gabapentin in vitro. After 9-12 hours of culture with or without gabapentin, FACS-purified CD34+ AML cells were subjected to transcriptome analysis.

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Differentiation of cultured THP-1 cells

Project description:Libraries constructed from cultured THP-1 cells receiving PMA treatment or not. Keywords: other

2004-11-18 | E-GEOD-1439 | biostudies-arrayexpress

Gene expression analysis of THP-1 cells co-cultured with platelet-like particles

Project description:Abstract. The role of platelets in hemostasis and thrombosis is clearly established; however, the mechanisms by which platelets mediate inflammatory and immune pathways are less well understood. Platelets interact and modulate the function of blood and vascular cells by releasing bioactive molecules. Although the platelet is anucleate, it contains transcripts that may mirror disease. Platelet mRNA is only associated with low-level protein translation, however, platelets have a unique membrane structure allowing for the passage of small molecules, leading to the possibility that its’ cytoplasmic RNA may be passed to nucleated cells. To examine this question, platelet-like particles with labeled RNA were co-cultured with vascular cells. Co-culture of platelet-like particles with activated THP-1, monocytic, and endothelial cells led to visual and functional RNA transfer. Post-transfer microarray gene expression analysis of THP-1 cells showed an increase in HBG1/HBG2 and HBA1/HBA2 expression which was directly related to the transfer. Infusion of wild-type platelets into a TLR2 deficient mouse model established in vivo confirmation of select platelet RNA transfer to leukocytes. By specifically transferring green fluorescent protein, it was also observed that external RNA was functional in the recipient cells. The observation that platelets possess the capacity to transfer cytosolic RNA suggests a new function for platelets in the regulation of vascular homeostasis. THP-1, treated with Pam3CSK4, were co-cultured with platelet-like particles for 12 hours and 24 hours then collected and processed for RNA isolation. We also included as control THP-1 untreated and THP-1 treated with Pam3CSK4. A total of 4 samples were analyzed by microarray for gene expression.

2012-05-17 | E-GEOD-38027 | biostudies-arrayexpress

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