Project description:Two groups of serologically confirmed ADV infected mink were used to analyze the association of Single Nucleotide Polymorphisms with Aleutian disease (AD) resistance. Group I (n=97) was comprised of disease susceptible (ADS) animals, with disease related hyper-gammaglobulinemia confirmed by the lowered albumin: IgG ratio (A: IgG); and Group II (n=97) contained disease resistant (ADR) animals with normal A: IgG ratio. The phenotypic assignment into the groups was done according to previously validated MALDI-TOF A: IgG ratio of high reproducibility in ADV infected animals. Illumina Hiseq 2500 sequencing was used to produce sequence libraries which were biocomputationally analyzed the genome wide spread SNPs where then associated with the disease susceptible and resistant groups of animals. There was a clear over-dominance for the GATOR complex protein NPRL3 isoform X6, with a very strong effect on the differences between the animals of ADS and the ADR groups. A minor effect was observed around the HLA complex, however scattered over more genes in this area. The Protocadherin Fat 3 (FAT3) or it neighborhood could also be regarded as a candidate aerea influencing the outcome of the infection. In the absence of vaccination, and in the light of eradication failures, the results provide the foundation for the development of genomic tests, as the basis for assisted breeding for disease resistance. Additionally, the results could be useful in investigations of genetic basis of the resistance of animal infections of major economic importance, e.g. African swine fever (ASF).

2020-12-22 | GSE163677 | GEO

American mink genome sequencing

Project description:American mink genome sequencing

| PRJEB16307 | ENA

An American mink transcriptome

Project description:An American mink (Neovison vison) transcriptome

| PRJEB1258 | ENA

American mink genome assembly

Project description:The first draft reference genome of the American mink (Neovison vison)

| PRJEB15663 | ENA

The first draft reference genome of the American mink (Neovison vison).

Project description: Not available

| S-EPMC5674041 | biostudies-literature

Metabolic Changes of Maternal Uterine Fluid, Uterus, and Plasma during the Peri-implantation Period of Early Pregnancy in Mice

Project description:Embryo implantation is a complex process which involves biochemical and physiological interactions between an implantation-competent blastocyst and a receptive uterus. However, the exact biochemical changes of uterine fluid, uterus, and plasma during peri-implantation remain unclear. This study aims to characterize the biochemical and metabolic changes that occur during the peri-implantation period of early pregnancy, using mice as an animal model. Gas chromatography-mass spectrometry was used to analyze the metabolite profiles of the uterus, uterine fluid, and maternal plasma at pre-implantation and implantation. The multivariate analyses, ANOVA and Tukey's HSD test, were applied to detect significant changes in metabolites and metabolic pathways. The metabolic networks were reconstructed in silico based on the identified metabolites and KEGG metabolic framework. Between pre-implantation day 1 and day 4, dramatic metabolic changes were observed in the uterine fluid that could be important for blastocyst development and protection against the harsh uterine environment. Palmitoleic acid, fumaric acid, and glutaric acid changed levels at day 4 in the uterus, suggesting that they may be associated with endometrial receptivity. Both the uterus and maternal plasma showed profound changes in cellular metabolism at the early implantation period, including upregulation of branched-chain amino acids and intermediates of one-carbon metabolism, an upregulation of glyoxylate and dicarboxylate metabolism, and downregulation of aerobic respiration; all of which could be involved in the regulation of the maternal-fetal interface, alternative nutrient utilization, and energy preservation for implantation as well as later placentation and fetal development to ensure successful embryo implantation.

2020-05-04 | MTBLS627 | MetaboLights

Neogale vison

Project description:Neogale vison Raw sequence reads

| PRJNA357306 | ENA