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Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

ABSTRACT: Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

PROVIDER: PRJNA548077 | ENA |

REPOSITORIES: ENA

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Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

Project description:Analysis of the transcriptome of THP-1 cells upon Huh7 cell-derived ectosomes incubation. Monocytic THP-1 cells were incubated with or without ectosomes (10 mg/ml) derived from scramble sequence transfection (Scramble Ecto) or PKM2 knocking down (PKM2 KD Ecto) Huh7 cells for 24 h. Results provide a novel insight into monocyte differentiation.

2022-06-08 | GSE132435 | GEO

RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

Project description:Purpose: The goals of this study are to compare the gene expression profile of Huh7 cells treated with different ectosomes or not and to evaluate the function of ectosomal Hexokianse 1 (HK1) derived from HSCs. Methods: Firstly, we treated different LX-2 HSCs with TGF-β (2 ng/mL) for 36 hours to activate HSCs, then ectosomes from LX-2 or HK1-KD LX-2 culture medium were isolated respectively by differential centrifugation processes. Secendly, Huh7 cells were preincubated with different ectosomes or not for 12 hours and then fresh medium for another 12 hours. Lastly, different groups of Huh7 cells were prepared for RNA-seq. Results: By Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed genes, a total of 124 pathways were found to be enriched in the ectosome-incubated Huh7 cells compared with the control Huh7 cells, while a total of 36 pathways were enriched in the ectosome-incubated Huh7 cells compared with the HK1-KD ectosome-incubated Huh7 cells.Gene set enrichment analysis (GSEA) also revealed enrichment of the N-glycosylation pathway in the ectosome-incubated Huh7 cells compared with the control Huh7 cells. Conversely, this pathway was negatively regulated in Huh7 cells incubated with HK1-KD HSC-derived ectosomes Conclusion: the transferal of HSC-derived ectosomal HK1 into HCC cells regulates the N-glycosylation level of Huh7.

2024-04-28 | GSE173632 | GEO

Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

Project description:ATACseq analysis of chromatin accessibility in Huh7 cells upon YFV capsid expression

2020-11-15 | GSE152623 | GEO

Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

Project description:Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

| PRJNA639846 | ENA

Proteins secreted by M1 and M2 macrophages derived from human buffy coat or THP-1 cells

Project description:Proteins secreted into the conditioned medium by human monocytic cell line, THP-1, or human buffy coat-derived monocytes polarised to either an M1 (incubation for 48 h with 100 ng/mL LPS and 20 ng/mL IFN-gamma) or M2 (incubation for 48 h with 20 ng/mL IL-4) phenotype. The conditioned medium was enriched for anionic molecules (including proteoglycans) by anion exchange chromatography prior to analysis.

2021-01-21 | MSV000086746 | MassIVE

Expression data from CF proband and parent PBMCs; expression data from THP-1 monocytes/macrophages incubated with CF proband and parent plasma

Project description:We used microarrays to look at differential gene expression in PBMC samples from proband, proband's parents, and healthy controls We used microarrays to look at the differential gene expression in THP-1 monocyte/macrophages after incubation with plasma from CF probands, proband's parents, healthy controls

2021-12-24 | GSE192523 | GEO

Soil incubation

Project description:Soil incubation Metagenome

| PRJNA509101 | ENA

Soil incubation

Project description:Soil incubation Metagenome

| PRJNA472852 | ENA

YFV Capsid changes to mRNA transcriptome of Huh7 cells

Project description:Genome-wide analysis of changes to mRNA transcript levels upon expression of YFV capsid in Huh7 cells

2020-11-15 | GSE152588 | GEO

DNA microarray analysis of Huh7 and JMJD5KO Huh7 cells.

Project description:To understand the roles of JMJD5 in liver cells, we performed DNA microarray analysis by using JMJD5KO Huh7 cells. We noticed that several transcriptional factors involved in differentiation to hepatocytes were down-regulated in JMJD5KO Huh7 cells compared to parent Huh7 cells..

2016-01-23 | GSE73491 | GEO

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