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Gene expression analysis of PJA1 knock down HepG2 cells or HepG2 cells treated with TGF-beta1.

ABSTRACT: Gene expression analysis of PJA1 knock down HepG2 cells or HepG2 cells treated with TGF-beta1.

PROVIDER: PRJNA548810 | ENA |

REPOSITORIES: ENA

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TGF-beta1-Induced Gene Expression Changes in HepG2 Cells

Project description:We here compared gene expression profiles in HepG2 cells upon stimulation with 1 ng/ml TGF-beta1 for 20 min, 1 hour, 2 hours, 4 hours, and 24 hours with untreated control cells. Experiments were done in three independent replicates. The goal of this study was to determine genes regulated by TGF-beta1.HepG2 cells were obtained from DSMZ (Braunschweig, Germany) and cell identity confirmed by STR profiling using the AmpFlSTR Identfiler Direct PCR Amplification kit (Life Technologies, Darmstadt, Germany). Gene expression profiles were compared at indicated time points after stimulation with TGF-beta (1 ng/ml) using the Human Gene 1.0 ST arrays (Affymetrix). In total 18 hybridizations are included in this series. 18 samples were hybridized to Affymetrix Human Gene 1.0 ST Array

2013-04-10 | E-GEOD-45945 | biostudies-arrayexpress

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Gene expression analysis of PJA1 knock down HepG2 cells or HepG2 cells treated with TGF-beta1.

Project description:Gene expression profiling was carried out in four (HepG2-shCtrl,HepG2-shPJA1, HepG2/Ctrl and HepG2/TGFb1 cells) different liver cancer cells. PJA1 is an E3 ligase that regulates TGF-beta signaling pathway. In this study, we report a major role of the PJA1-mediated desregulation of TGF-beta/Smad3 in liver cancers . To determine the mechanism for the oncogenic function of PJA1 in TGF-β signaling transduction, we analyzed gene profiles the cells of knockdown PJA1 in HepG2 cells and the cells treated with TGFb1 in HepG2.

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Citrullination of latency-associated peptide of TGF beta1

Project description:Enzymatic citrullination of latency-associated peptide of TGF beta1 by PAD2

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RNA-seq of human lung fibroblasts with and without TGF-beta1

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Genetic modifier of TGF-beta1 stimulated pulmonayr fibrosis

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