Project description:To identify female sex pheromone biosynthesis genes by differential expression between males and females. Female pheromone gland tissue and male abdominal tip tissue was compared by RNA-Seq of cDNA. Transcripts were assembled using Trinity and differential expression analysis done with RSEM.
Project description:Silkworms show a reproductive behavior induced by sex pheromone. To elucidate the neral mechanism of sex pheromone induced sexual behavior in the silkworm, we attempted to use the neural activity-induced gene as a neural activity marker. Since no neural activity-induced gene was identified in the silkworm, we conducted screening of neural activity-induced gene using the male silkworm brain. By the screening, we identified Bhr38 as a novel neural activity-induced gene, and succeded to comprehensively map the active neruons in the silkworm brain in response to the sex pheromone exposure. Further, we found that Dhr38, the Drosophila homologue of Bhr38, also expressed in a neural activity dependent manner. These results strongly suggest that Hr38 is a highly conserved neural activity-induced gene.
Project description:Calcineurin-regulated Phosphopeptide(30 min) and CaN-regulated dephosphopeptide(10 min) researchs used Itraq method in Pheromone gland of Helicoverpa armigera
