ABSTRACT: Barcoding project of insect and plant trypanosomatids availables at Fiocruz Protozoa Culture Collection http://colprot.fiocruz.br/ Phenotype or genotype
Project description:Three strains, P. zopfii genotype 1 (SAG 2063), P. zopfii genotype 2 (SAG 2021) and P. blaschkeae (SAG 2064) from the culture collection at the Institute of Animal Hygiene and Environmental Health, Freie Universität Berlin, Germany were cultured six times independently at 37°C in Sabouraud dextrose liquid medium until OD600 measurement reached 0.6 to 0.9. Following the cell harvest by brief centrifugation, the cells were lysed and label free quantitative proteomics analysis were carried out to investigate the differences between these strains.
Project description:Interventions: Intravenous adminstration of CPT-11 100 mg/m2 on Days 1, 8, and 15, every 4 weeks
Primary outcome(s): 1)Tumor response rate 2)Genotype-phenotype association (CYP3A4,CES1,2, UGT1A1, ABCG2, ABCB1, and ABCC1,2 vs toxicity), mutation -phenotype association (TOP1A, ABCG2 vs tumor response), and expression-phenotype association (TOP1A, ABCG2 vs tumor response)
Study Design: Single arm Non-randomized
Project description:RNA-seq from human tissue samples from the Genotype-Tissue Expression (GTEx) Project (http://www.gtexportal.org/home/). This dataset is part of the TransQST collection.
