Project description:We measured mRNA abundance in the seedling leaves of the barley genotypes Golden Promise and Morex and in F1 hybrids generated using either Golden Promise as a maternal genotype and Morex as paternal or other way around. 3 biological replicates each, total 12 chips.

2007-12-31 | E-TABM-110 | biostudies-arrayexpress

Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise

Project description:Salt Stress response of salt-tolerant genotype Golden Promise compared to Maythorpe Barley1 GeneChip was used to find differential expression between two barley genotypes under control and salt stress conditions at vegetative stage of growth Keywords: genotype and treatment comparison

2007-03-31 | GSE6325 | GEO

Transcription profiling of seedling leaves from 8 barley genotypes: Morex, Steptoe, Golden Promise, Optic, Haruna Nijo, Barke, OWB-D and OWB-R

Project description:We measured mRNA abundance in the seedling leaves of 8 barley genotypes; Morex, Steptoe, Golden Promise, Optic, Haruna Nijo, Barke, OWB-D and OWB-R. 3 biological replicates each, total 24 chips.

2007-12-31 | E-TABM-113 | biostudies-arrayexpress

RNA-seq of drought response and drought recovery in barley (cv. Golden Promise) on a lysimeter platform.

Project description:Determination of changes in expression of genes and pathways as a consequence of drought treatment and following rewatering in barley cv. Golden Promise. RNAs were collected following stomatal closure (theta-critical point) and following rewatering from leaves as well as under fully watered conditions. The plants were grown under greenhouse conditions.

2024-02-01 | E-MTAB-12732 | biostudies-arrayexpress

Transcriptomic changes in the crown (stem base) of barley (Hordeum vulgare L. cv. Golden Promise) during crown-root initiation and development

Project description:Purpose: The goal of this study was to investigate the mechanisms involved in the initiation and development of crown-roots (CRs) in barley and to estimate the role of cytokinins (CKs) in this process. Method: stranded libraries were obtained from RNA extracted from the stem base of 1 day-after-germination (DAG) and 10DAG-seedlings of wild-type (WT) and AtCKX-overexpressing barley lines (OE-CKX). OE-CKX lines have a reduced content of endogenous CKs and are characterized by a higher number of CRs. Libraries were deep sequenced on Illumina HighSeq platform. Each sample was investigated in three independent biological replicates. Results: Using a data analysis workflow optimized for barley, we identified more than 4000 transcripts differentially expressed in the stem base of 1DAG and 10DAG-seedlings. Expression as determined by RNA-seq was validated by real-time PCR. Our data were compared to the transcriptomic profiling obtained from rice and we were able to identify genes potentially involved in the initiation/development of CRs in barley. Also the use of the transgenic line with altered endogenous CK content allowed us to conclude about the role of CKs in the process. Conclusions: Our study represents the first analysis aiming to understand the initiation and development of CRs in barley.

2016-11-01 | GSE87737 | GEO

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