Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages

ABSTRACT: Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages

PROVIDER: PRJNA549471 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages

Project description:Inner ear cochlear spiral ganglion neurons (SGNs) transmit sound information to the brainstem. Recent single cell RNA-Seq studies have revealed heterogeneities within SGNs. Nonetheless, much remains unknown about the transcriptome of SGNs, especially which genes are specifically expressed in SGNs. To address these questions, we needed a deeper and broader gene coverage than that in previous studies. We performed bulk RNA-Seq on mouse SGNs at five ages, and on two reference cell types (hair cells and glia). Their transcriptome comparison identified genes previously unknown to be specifically expressed in SGNs. To validate our dataset and provide useful genetic tools for this research field, we generated two knockin mouse strains: Scrt2-P2A-tdTomato and Celf4-3xHA-P2A-iCreER-T2A-EGFP. Our comprehensive analysis confirmed the SGN-selective expression of the candidate genes, testifying to the quality of our transcriptome data. These two mouse strains can be used to temporally label SGNs or to sort them.

2020-01-07 | GSE132925 | GEO

Spiral ganglion subtype specification occurs prior to birth in the mouse

Project description:Little is known about the timing and factors which regulate the specification of neuronal subtypes in the cochlear spiral ganglion. Here we collect Spiral Ganglion Neurons (SGNs) from the mouse across four developmental timepoints (E14, E16, E18 and P1) in order to transciptionally profile their development.

2022-11-22 | GSE195500 | GEO

Transcriptional profiling of cochlear spiral ganglion neurons and lateral wall cells

Project description:Transcriptional profiling of cochlear spiral ganglion neurons and lateral wall cells

| PRJNA705914 | ENA

Expression of Wnt Receptors in Adult Spiral Ganglion Neurons: Fzd 9 Located at Growth Cones of Regenerating Neurites

Project description:The fidelity of sound transmission by cochlear implants in patients with sensorineural hearing loss could be greatly improved by increasing the number of frequency channels. This could be achieved by stimulating and guiding neurite outgrowth to reduce the distance between the implant's electrodes and the remnants of the spiral ganglion neurons. However, little is known about signaling pathways, besides those of neurotrophic factors, that are operational in the adult spiral ganglion. To systematically identify neuronal receptors for guidance cues in the adult cochlea, we conducted a genome-wide cDNA microarray screen with two-month-old CBA/CaJ mice. A meta-analysis of our data and those from older mice in two other studies revealed the presence of neuronal transmembrane receptors that represent all four established guidance pathways—ephrin, netrin, semaphorin, and slit—in the mature cochlea as late as 15 months. In addition, we observed the expression of all known receptors for the Wnt morphogens, whose neuronal guidance function has only recently been recognized. In situ hybridizations located the mRNAs of the Wnt receptors frizzled 1, 4, 6, 9, and 10 specifically in adult spiral ganglion neurons. Finally, frizzled 9 protein was found in the growth cones of adult spiral ganglion neurons that were regenerating neurites in culture. We conclude from our results that adult spiral ganglion neurons are poised to respond to neurite damage, owing to the constitutive expression of a large and diverse collection of guidance receptors. Wnt signaling, in particular, emerges as a candidate pathway for guiding neurite outgrowth towards a cochlear implant after sensorineural hearing loss.

2009-09-16 | GSE12810 | GEO

RNAseq of A/J mice spiral ganglion derived neurospheres vs differentiated auditory neurons

Project description:Sphere-forming progenitor cells can be isolated from the fetal and adult mammalian inner ear and give rise to inner ear specific cell types in vitro. Here, we provide phenotypical and functional characterization of a new pool of auditory progenitors as sustainable source for sphere-derived auditory neurons. The so-called phoenix auditory neuroprogenitors, isolated from the A/J mouse spiral ganglion, exhibit nearly unlimited intrinsic self-renewal properties (beyond 40 passages). At any passage, phoenix spheres can be efficiently differentiated by withdrawing growth factors into mature spiral ganglion cells, expressing both neurons and glial cells phenotypic markers and exhibiting similar functional properties as mouse spiral ganglion explants and human sphere-derived spiral ganglion cells. The present dataset includes RNAseq-based transcriptome analysis of phoenix auditory neurons following 7 days of differentiation. mRNA levels in differentiated cells are expressed relatively to neuroprogenitor spheres of equivalent passage (paired samples at passage 12, 21 and 36)

2020-08-29 | E-MTAB-9441 | biostudies-arrayexpress

Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages.

Project description: Not available

| S-EPMC7299348 | biostudies-literature

Transcriptional profiling of cochlear spiral ganglion neurons and lateral wall cells

Project description:In order to characterize the response to noise of various cell types in the inner ear, the cochleae from wild-type mice, naive or subjected to a 2h, 105 dB SPL noise insult, were dissected out. To further examine spiral ganglion neurons in more detail, non-neuronal populations were removed in one set of samples. In another set of samples, the lateral wall tissue was isolated to better understand how cells from the stria respond to noise insults. The resulting tissue was then dissociated to obtain a single cell suspension, and profiled by single cell RNA-seq.

2021-09-08 | GSE168041 | GEO

Microarray expression profiling of the spiral ganglion of hearing and deafened rats

Project description:Gene expression profiling of apical and basal halves of the spiral ganglion of hearing and kanamycin-deafened rats at P32 and P60 was performed using microarray analysis. The data are further described in Rahman MT, Bailey EM, Gansemer BM, Pieper AA, Manak JR, and Green SH. Anti-inflammatory compounds improve spiral ganglion neuron survival after aminoglycoside-induced hair cell loss in rats.

2023-04-26 | GSE190146 | GEO

Gene expression profiling in a mouse model of spiral ganglion neuron degeneration

Project description:The goals of this study are to identify transcripts that participate in the degeneration of spiral ganglion neurons.

2021-08-30 | GSE182978 | GEO

Single-cell RNA-seq of spiral ganglion neurons from wildtype and Vglut3-/- mice

Project description:Molecular heterogeneity among spiral ganglion neurons (SGNs) in the mouse cochlea was investigated in two genetic backgrounds: 1) wildtype, 2) Vglut3-/-, which lack inner hair cell-driven glutamatergic activation of SGNs.

2018-08-06 | GSE114997 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data