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D39 wild-type vs D39 ΔplcR in GM17 medium

ABSTRACT: D39 wild-type vs D39 ΔplcR in GM17 medium

PROVIDER: PRJNA549688 | ENA |

REPOSITORIES: ENA

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D39 wild-type vs D39 ΔplcR in GM17 medium

Project description:Comparison of the Streptococcus pneumoniae D39 wild-type vsD39 ΔplcR (SPD1745) in GM17medium

2019-07-31 | GSE133010 | GEO

Streptococcus pneumoniae D39 spxB- mutant vs. D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was ≥ 2, ≤ 0.5, with a Bayes p ≤ 0.00001 and when at least 7 measurements were available.

2013-08-08 | GSE45971 | GEO

adcR mutant vs wild type D39

Project description:Comparison of the Streptococcus pneumoniae D39 adcR mutant vs D39 wild type in CDM plus 0.2mM Zn One condition design comparison of two strains including a dye swap

2011-05-13 | E-GEOD-29234 | biostudies-arrayexpress

Streptococcus pneumoniae D39 spxB- mutant vs. D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was M-bM-^IM-% 2, M-bM-^IM-$ 0.5, with a Bayes p M-bM-^IM-$ 0.00001 and when at least 7 measurements were available. One condition design comparision of two strains including a dye swap.

2013-08-08 | E-GEOD-45971 | biostudies-arrayexpress

cps wild type vs cps mgtA mutant

Project description:Comparison of the Streptococcus pneumoniae D39 cps mgtA mutant vs cps wild type

2011-05-05 | GSE26154 | GEO

D39 wild-type vs D39 ÎadcR In CDM with 0.3 mM Ni2+

Project description:By using the transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB and phtE) and the PsaR regulon (pcpA, prtA and psaBCA) were highly upregulated in the presence of Ni2+. We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic mobility shift assays and transcriptional lacZ-reporter studies, and demonstrate that Ni2+ is directly involved in the derepression of the AdcR regulon via the Zn2+-dependent repressor AdcR, and has an opposite effect on the expression of the AdcR regulon as compared to Zn2+. Comparison of the Streptococcus pneumoniae D39 wild-type vs D39 ÎadcR in CDM Plus 0.3 mM Ni2+ Two condition design comparison of Wild-type strain vs mutant strain including a dye swap

2015-11-20 | E-GEOD-73851 | biostudies-arrayexpress

Comparison of the Streptococcus pneumoniae D39 lacR Mutant compared to D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 lacR mutant compared to D39 wild type in M17 medium+ 0.5 % (w/v) Glucose (GM17) Two condition design comparison of Wild-type strain including a dye swap

2014-06-20 | E-GEOD-58183 | biostudies-arrayexpress

Comparison of the Streptococcus pneumoniae D39 ulaR Mutant compared to D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 ulaR mutant compared to D39 wild type in M17 medium + 10mM ascorbic acid (AM17) One condition design comparision of two strains including a dye swap

2014-10-28 | E-GEOD-61649 | biostudies-arrayexpress

Comparison of the Streptococcus pneumoniae D39 lacT Mutant compared to D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 lacT mutant compared to D39 wild type in M17 medium+ 0.5 % (w/v) Lactose (LM17) One condition design comparision of two strains including a dye swap

2014-06-20 | E-GEOD-58182 | biostudies-arrayexpress

Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type treated with 1mM to with 0mM H2O2

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in THY medium till OD620=0.25 and then treated with or without 1mM H2O2 for 40mins.

2017-07-27 | GSE65155 | GEO

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