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Single Cell mRNA Sequencing of Pca-associated CD14+CD11b+ macrophages

ABSTRACT: Single Cell mRNA Sequencing of Pca-associated CD14+CD11b+ macrophages

PROVIDER: PRJNA549935 | ENA |

REPOSITORIES: ENA

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Single Cell mRNA Sequencing of Pca-associated CD14+CD11b+ macrophages

Project description:Purpose: Androgen receptor (AR) is a crucial modulator of prostate cancer (PCa) cells behaviour, and AR expression has been found in several stromal cells, including macrophages, however its role in these cells in largely unknown. In this study, we described the molecular mechanims and the functional implications of AR activation and blockade in macrophages in relation to PCa progression. Results: Analysis showed the transcriptomic landscape of PCa-associated macrophages Conclusions: Our study represents the first detailed analysis of AR molecular function in Pca-associated macrophages

2019-06-21 | GSE133094 | GEO

Transcription profiling by array of human serum-free fibrocytes, serum-containing fibrocytes, CD14++CD16- Monocytes, CD14++CD16+ Monocytes, CD14+CD16++ Monocytes and macrophages

Project description:Serum-free Fibrocytes, Serum-containing Fibrocytes, CD14++CD16- Monocytes, CD14++CD16+ Monocytes, CD14+CD16++ Monocytes, Macrophages were all generated from up to 3 biological replicates from each of 3 separate donors. RNA was extracted (Ambion RNAqueous), labelled with cy3, mixed with cy5 labelled human reference (Stratagene), and hybridised to slides printed with Human AROS v4.0 oligonucleotides (Operon). Slides were scanned using a Perkin Elmer GX plus, and the data then normalised with GEPAS v4.0 and collated. Final data analysis was carried out using TMEV 4.0. SAM was performed using a 0.1% FDR. PCA were plotted from this list, and interrogation carried out using DAVID to determine pathway enrichment.

2011-01-01 | E-MEXP-2811 | biostudies-arrayexpress

Response of CD14-derived macrophages to LPS

Project description:Response of CD14-derived macrophages to LPS

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Response of CD14-derived macrophages to LPS

Project description:We releaved the transcriptomic changes after LPS stimulation of CD14-derived macrophages from three donors.

2020-12-15 | GSE163165 | GEO

Gene profile of human CD14+ monocytes and monocyte-derived macrophages

Project description:Comparison of the transcriptome of CD14+ human monocytes and CD14+ human monocyte-derived macrophages generated in the presence of M-CSF (M-MØ) or GM-CSF (GM-MØ).

2022-03-24 | GSE188278 | GEO

CD11b restricts alveolar macrophage activation during pneumococcal pneumonia and prevents excessive lung inflammation

Project description:Bacterial lung infections are associated with strong infiltration of CD11b+ myeloid cells, which limit life-threatening disease, but also severely damage lung tissue. In a murine lung infection model with Streptococcus pneumoniae, we found intrinsic upregulation of CD11b on resident alveolar macrophages. Such CD11b expression was associated with transcriptomic and proteomic adaptations by alveolar macrophages, leading to the identification of specific molecules and pathways that depended on CD11b. In the absence of CD11b, the antimicrobial defense of alveolar macrophages was strongly reduced, and the production of neutrophil-recruiting chemokines was more pronounced. Moreover, CD11b expression limited the infection and prevented excessive alveolar damage. In conclusion, our study provides detailed molecular insights into the alveolar macrophage-specific immune response to Streptococcus pneumoniae lung infection and reveals profound CD11b-dependent alterations that are critical for effective antimicrobial immunity, neutrophil recruitment, and prevention of alveolar damage.

2023-10-08 | PXD035269 | Pride

Regulatory role of multinucleated giant cells derived from placental CD14+ macrophages: Pathophysiological implications

Project description:The role of placental macrophages is largely ignored in the success of pregnancy. We found that CD14+ macrophages purified from at-term placentas spontaneously matured into multinucleated giant cells (MGCs). MGCs lost the expression of CD14 and co-inhibitory molecules, such as Programmed cell Death-Ligands 1 and 2. Although MGCs kept phagocytosis and property to produce ROS, their inflammatory potential measured by TNF/IL-10 imbalance and activation of p38 MAPK and NF-M-NM-:B was blunted without being associated with M2 phenotype. The investigation of gene expression revealed the enrichment with categories related to inflammation, apoptosis and canonical functions of macrophages in MGCs. Whereas most of the genes associated with inflammation and immune responses were down-modulated, those such as VEGFC or associated with matrix remodeling were specifically up-regulated in MGCs. Importantly, we found that patients with preeclampsia or chorioamnionitis, two inflammatory and infectious pathologies, respectively, that affect placentas, were unable to generate MGCs. Taken together, these results suggest that MGCs represent a new way to regulate the inflammatory and cytocidal activity of placental macrophages in a context that imposes contradictory constraints, such as semi-allograft acceptance and defense against aggression. The dysregulation of MGC formation may be associated with placental inflammatory and infectious pathologies. Placental macrophages CD14+ were cultured for 9 days to form multinucleated giant cells (MGC). The transcriptome of MGCs was compared to the transcriptome of placental macrophages (CD14+)

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RNA-seq of small intestinal macrophages, neutrophils, CD103-CD11b+cDC and cryptopatch and ILF associated mononuclear phagocytes (CIA-MPh)

Project description:Cryptopatches (CP) and isolated lymphoid follicles (ILF) are evolutionary ancient lymphoid structures found in the intestines of all vertebrates. These structures are demarcated by a poorly characterised subset of mononuclear phagocytes (MPh), called CP/ILF-associated (CIA)-MPh. CIA-MPh expressed very high levels of lysozyme M (LysM), but they did not express Ly6G or CD64, normally associated with LysM-expressing neutrophils or macrophages, respectively. In order to understand the ontogeny and function of these newly identified MPh subset, we performed genome-wide transcriptional profiling of CIA-MPh and other cell types expressing LysM (i.e., neutrophils and macrophages) in the small intestine as well as of CD11b+ CD103- cDC2. Using Lyz2-GFP reporter mice, we developed a sorting strategy allowing us to highly purify neutrophils, macrophages, CD11b+ CD103- cDC2 and CIA-MPh. The RNA of each sample was extracted using the ImmGen protocol with phase lock tubes. The libraries were prepared using the SMARTer Ultra Low Input RNA kit (Clontech) according to the manufacturer’s instructions. The material was enriched for poly-A sequences and single-end Next Generation Sequencing was performed on a HiSeq 2500 machine (Illumina).

2020-11-24 | E-MTAB-9505 | biostudies-arrayexpress

Pleural CD14+ monocytes/macrophages of healthy adolescents showa high expression of metallothionein family genes

Project description:High quality CD14+ monocytes/macrophages (plMo/Mφ) were used for RNA-sequencing (RNA-seq) in comparison with human monocyte-derived macrophages (MDM) natural (MDM-0) or IL-4-polarized(MDM-IL4)

2023-09-06 | GSE217995 | GEO

Quantitative Temporal in vivo Proteomics (QTiPs) deciphers the transition of inflammatory CD11b+,Ly6G-,Ly6Chigh myeloid cells into M2-like macrophages

Project description:Phenotypic transition of myeloid cells into distinct lineages in vivo is important in pathogen response. To monitor immune cell phenotype transitions in vivo, we developed a quantitative temporal in vivo proteomics (QTiPs) platform, performing multiplexed (10-plex) tandem-mass-tag (TMT)-based mass spectrometry on sorted cells collected from their in situ microenvironment during infection. We temporally characterized a poorly understood, virus-driven CD11b+,Ly6G-,Ly6Chigh-low myeloid cell population throughout an acute phase of infection in both the site of infection and bone marrow. QTiPs, in combination with phenotypic, functional and metabolic analyses, elucidated a pivotal role for inflammatory CD11b+,Ly6G-,Ly6Chigh-low cells in anti-viral immune response and viral clearance. Most importantly, the highly time-resolved QTiPs dataset showed the transition of CD11b+,Ly6G-,Ly6Chigh-low cells into M2-like macrophages which displayed increased antigen presentation capacities and bioenergetic demands late in infection. Our QTiPs approach precisely captures myeloid cell-macrophage transition in this population, and it is a novel platform for measuring temporospatial proteome transitions in vivo.

2017-08-09 | PXD005064 | Pride

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