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H3K27me3-rich genomic regions can function as silencers to repress gene expression via chromatin interactions

ABSTRACT: H3K27me3-rich genomic regions can function as silencers to repress gene expression via chromatin interactions

PROVIDER: PRJNA550207 | ENA |

REPOSITORIES: ENA

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H3K27me3-rich genomic regions can function as silencers to repress gene expression via chromatin interactions

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Project description:Enhancers and silencers often depend on the same transcription factors (TFs) and are imperfectly distinguished from each other by genomic assays of TF binding or chromatin state. To identify sequence features that define enhancers and silencers, we assayed massively parallel reporter libraries of genomic sequences targeted by the photoreceptor TF CRX and found instances of enhancer, silencer, or no activity. Both enhancers and silencers contain more TF motifs than inactive sequences, but enhancers contain motifs from a more diverse collection of TFs. We developed a measure of information content that describes the number and diversity of motifs in a sequence and found that, while both enhancers and silencers depend on CRX motifs, enhancers have higher information content. Our results indicate that enhancers contain motifs for a diverse but degenerate collection of TFs, while silencers depend on a smaller and less diverse collection of TFs.

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