Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing of Wild Type and Gata2-/- LSCs

ABSTRACT: Next Generation Sequencing of Wild Type and Gata2-/- LSCs

PROVIDER: PRJNA550443 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Next Generation Sequencing of Wild Type and Gata2-/- LSCs

Project description:Gata2, a zinc finger TF, is essential for the generation and survival of HSCs in the embryo and has been implicated in the pathogenesis of AML, yet the requirement for Gata2 in adult HSCs and LSCs remains unclear. Using a conditional mouse model where Gata2 was deleted specifically in hematopoietic cells, we show that knockout of Gata2 leads to a rapid and complete cell-autonomous loss of adult HSCs. In Meis1a/Hoxa9 driven AML, deletion of Gata2 impedes maintenance and self-renewal of LSCs. We then performed RNA-seq from sorted control and Gata2 KO LSCs (CD45.2+ c-Kit+) after pIpC treatment in transplanted mice.

2019-06-25 | GSE133245 | GEO

Next Generation Sequencing of Wild Type and Gata2+/- HSCs

Project description:Gata2, a zinc finger TF, is essential for the generation and survival of HSCs in the embryo and has been implicated in the pathogenesis of AML, yet the requirement for Gata2 in adult HSCs and LSCs remains unclear. Using a conditional mouse model where Gata2 was deleted specifically in hematopoietic cells, we show that knockout of Gata2 leads to a rapid and complete cell-autonomous loss of adult HSCs. We then performed RNA-seq in sorted HSCs (LSK CD48- CD150+) from control and Gata2+/fl;Vav-iCre+ 8-to-10-week old mice.

2019-06-25 | GSE133248 | GEO

Next Generation Sequencing of Wild Type and Gata2+/- HSCs

Project description:Next Generation Sequencing of Wild Type and Gata2+/- HSCs

| PRJNA550447 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of effect of knockdown of GATA2 on AR binding sites

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare AR binding activity in LNCaP cells with and without knockdown of GATA2. Methods: LNCaP cells between passage number 32-34 were used for assay. Cells are transfected with GATA2 specific or nonspecific siRNA and ChIP was performed, the ChIP producted was further used to generate library with illumina ChIP-seq kit. Hi-seq 2500 was used for sequencing and the data was analyzed by MACs for peaks. Results: GATA2 knockdown lead to changes of AR binding activity , in most AR binding sites, AR shows decreased bindig activity. Only small percent sites show increased binding. Conclusions: Our study represents the first detailed analysis of the relationship between GATA2 and AR binding in whole genomic DNA.These results demostrate GATA2 play a critical role in AR activity in prostate cancer. LNCaP cells was used as cell model were treated with specific GATA2 siRNA.Library was sequenced using Illumina HI-seq 2500.

2014-01-21 | E-GEOD-52725 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of effect of knockdown of GATA2 on AR binding sites

2014-01-21 | GSE52725 | GEO

Next Generation Sequencing of Wild Type and Zeb1-/- CLP

Project description:Next Generation Sequencing of Wild Type and Zeb1-/- CLP

| PRJNA643569 | ENA

Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

Project description:Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

| PRJNA643571 | ENA

Next Generation Sequencing Facillitates Quantitative Analysis of Wild Type and snrk1a1/a2 mutant Transcriptomes

Project description:Next Generation Sequencing Facillitates Quantitative Analysis of Wild Type and snrk1a1/a2 mutant Transcriptomes

2021-12-31 | GSE183118 | GEO

Next generation sequencing for quantitative analysis of wild type and Y41F transcriptomes

Project description:Next Generation Sequencing (NGS) on total RNA of wild type (WT) and Y41F mutant. The goal of this study is to compare the transcriptome profile of Y41F mutant to WT cells.

2017-12-06 | GSE102125 | GEO

Next Generation Sequencing of Wild Type and Nlrp6-/- T cells

Project description:Next Generation Sequencing of Wild Type and Nlrp6-/- T cells

| PRJNA478531 | ENA