Project description:IL-15-responsive CD122+ macrophages (CD122+Macs) develop in the uterus during normal pregnancy. We aimed to understand the signals driving macrophages to adopt this fate. We aimed to determine how CD122+Macs and conventional uterine macrophages differ transcriptionally.
Project description:Exploration of proteome differences between CD45+ and CD45- cell types in renal cell carcinoma tumors and normal adjacent tissue patient samples.
Project description:Uterine natural killer(NK) cells play a crucial role in pregnancy by promoting spiral artery remodeling and secreting fetal growth factors.Uterine trNK cells before pregnancy and during pregnancy (gd6.5, gd8.5 and gd11.5) were sorted, then applied into bulk RNA-sequence to reveal the features at different timepoints. Similarily, uterine trNK cells with different treatment in vitro were also sorted for bulk RNA-sequence to identify the role of IL-21-STAT3 signaling. To interpret the differentiation trajectory of uterine trNK cells, CD45+NKp46+NK1.1+ cells in wild-type virgin mice, wild-type gd8.5 mice, Il21r-/- gd8.5 mice and Il21r-/- gd8.5 mice with Caspase3 inhibitor are sorted.Applying them to Single-Cell RNA sequence, a differentiation trait of uterine NK cells during pregancy was revealed and IL-21 significantly promoted this process at initial stage.
Project description:Splenic NK cells were enriched and cultured in either low dose (5-10 ng/ml) or high dose (100 ng/ml) IL-15. Naïve NKs are freshly enriched NKs, obtained the day of the anti-NK1.1 stimulation. Cells were either left unstimulated or were stimulated via plate-bound anti-NK1.1
Project description:Natural killer (NK) cells have differential expression of inhibitory and activating receptors. NK cells that express inhibitory receptors with high binding affinity to self are considered licensed and normally functional cells, while those with low affinity are unlicensed are considered hyporesponsive. Microarray analysis was performed to better understand gene expression differences between these two subpopulations of NK cells after culturing the NK cells with IL-2 from splenocytes isolated from C57BL/6 mice and then sorting for CD3-CD122+Ly49G2+Ly49C/I- or CD3-CD122+Ly49G2-Ly49C/I+. Gene fold expression differences were compared between the Ly49G2+ and Ly49C/I+ NK cells to better understand the functional roles and characterisitics of these cells. A total of 6 samples were analyzed, 3 for the CD3-CD122+Ly49G2+Ly49C/I- NK cells and 3 for the CD3-CD122+Ly49G2-Ly49C/I+. Samples were obtained from C57BL/5 splenocytes that were T cells depleted through anti-Thy1.2 and complement mediated depletion and cultured for 7 days with IL-2. Fold changes greater than 1.5 were recorded.
Project description:The NK cell pool is composed of distinct NK cell subsets with divergent phenotypic and functional features. In order to determine whether DX5- and DX5+ NK cells from murine livers represent different NK cell subsets, DX5- and DX5+ liver NK cells of adult mice were respectively sorted for gene expression analysis using the Affymetrix GeneChip Mouse Genome 430 2.0 arrays. DX5-NK1.1+CD3- cells and DX5+NK1.1+CD3- cells were sorted from the liver of naive B6 wild-type mice. RNA of each sample was then extracted and hybridized on Affymetrix microarrays to detail differences between DX5- and DX5+ liver NK cells in gene expression.