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Plasma circulating miRNA profiling in polymicrobial sepsis by high throughput sequencing

ABSTRACT: Plasma circulating miRNA profiling in polymicrobial sepsis by high throughput sequencing

PROVIDER: PRJNA552351 | ENA |

REPOSITORIES: ENA

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Plasma circulating miRNA profiling in polymicrobial sepsis by high throughput sequencing

Project description:Aim: To explore the potentially important role of circulating miRNAs in systemic inflammation during sepsis. Method: 8 to 10-week old C57BL/6 mice underwent cecal ligation and puncture (CLP) or laparotomy (Sham). Plasma RNA was isolated using Trizol LS reagent 24h post-surgery. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform.

2020-04-12 | GSE133733 | GEO

Plasma exosomal miRNAs microarray in polymicrobial sepsis

Project description:Plasma exosomal miRNAs microarray in polymicrobial sepsis

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Polymicrobial sepsis-induced Myocardial Depression Transcriptional Profile

Project description:To describe the transcriptional changes associated with polymicrobial-sepsis induced myocardial depression in wild type and iNOS deficient mice. Keywords: myocardium, contractility, differential gene expression, nitric oxide synthase, infection We compared the transcriptional profile of C57/BL6 WT mice and congenic B6 129P2-Nos2tm1Lau/J mice after 48 hrs of polymicrobial sepsis induced by caecal ligation and perforation. 48 hours after surgery, mice were anaesthetised (intraperitoneal 100 mg/kg ketamine and 10 mg/kg xylazine). The right common carotid artery was cannulated (Millar Mikro-Tip pressure transducing catheter: 1.4F sensor, 2F catheter; Houston TX). Pressure tracings from the aorta and left ventricle were recorded (SonoLAB software; Sonometrics Corp., London Ontario Canada) and analysed using Cardiosoft and Origin 6.0 (Sonometrics Corp., and Microcal Software, Northampton MA). The heart was removed, emptied of blood, and snap frozen.

2010-11-17 | E-GEOD-9667 | biostudies-arrayexpress

Polymicrobial sepsis-induced Myocardial Depression Transcriptional Profile

2010-11-17 | GSE9667 | GEO

The role of ferritin heavy chain (Fth) in polymicrobial sepsis.

Project description:We systematically assessed the transcriptomic changes of livers of MxCreFthD/D vs. Fthlox/lox mice after induction of polymicrobial sepsis using Cecal Ligation and Puncture. Data indicates a distinct set of genes differentially regulated between MxCreFthD/D and Fthlox/lox mice after sepsis induction reflecting altered iron and glucose metabolism.

2017-08-15 | GSE92703 | GEO

small RNA sequencing of plasma miRNA from sepsis patients

Project description:Purpose: The aim of this study is to compare the plasma miRNA profile between healthy control and sepsis patients Methods: Plasma from healthy control and sepsis patients were used in the study. Total RNA was isolated from equal volume of plasma using Trizol LS. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform.

2020-05-04 | GSE149764 | GEO

Innate response activator B cells are sentinels that guard against polymicrobial sepsis

Project description:Innate immunity is fundamental to recognition and clearance of bacterial infection. The relevant cells and molecules that orchestrate an effective response, however, remain incompletely understood. Here we describe a previously unknown population of B cells, which we have named innate response activator (IRA) B cells that recognize bacteria directly through TLR-4-MyD88 and protect against polymicrobial sepsis. IRA-B cells have a unique IgM high CD23 low CD43 + CD93+ GM-CSF+ signature, develop and diverge from B1a B cells, require BAFFR, and adhere to tissue via VLA-4 and LFA-1. B cell subsets are sorted from the spleen and peritoneum of C57BL/6 mice that were given intraperitoneal injections of LPS once daily for four days.

2012-02-07 | E-GEOD-32372 | biostudies-arrayexpress

Transcriptomic profiles of blood in murine polymicrobial sepsis

Project description:Transcriptional effects in circulating leukocytes from mice challenged to peritoneal contamination and infection (PCI) compared to sham (vehicle control). We gratefully acknowledge the BMBF grant for the Center for Sepsis Control and Care (CSCC) for this study

2017-09-01 | GSE36608 | GEO

Plasma exosomal miRNAs microarray in polymicrobial sepsis

Project description:Exosomal RNA isolated from plasma was successfully profiled with a total of 65 immunology-related miNRAs in 13 mouse samples

2018-07-10 | GSE116829 | GEO

Profiling circulating microRNA expression in experimental sepsis by cecal ligation and puncture

Project description:To profile the expression of circulating microRNAs (miRNAs) of mice in experimental sepsis by cecal ligation and puncture (CLP), the whole blood samples were obtained from C57BL/6 mice at 4, 8, and 24 h following CLP for miRNA expression analysis using a miRNA array (The Mouse & Rat miRNA OneArray® v3). Briefly, mice were anesthetized with a combination of ketamine and xylazine as the anesthetic/analgesic agents and a midline abdominal incision was made. The cecum was mobilized, ligated in the middle of cecum below the ileocecal valve, punctured once with a 21 G needle, and a little stool was squeeze out of the cecum to induce polymicrobial peritonitis. The abdominal wall was closed in two layers. Sham-operated mice underwent the same procedure, including opening the peritoneum and exposing the bowel, but without ligation and needle perforation of the cecum. the whole blood samples were obtained from C57BL/6 mice at 4, 8, and 24 h following CLP for miRNA expression analysis using a miRNA array (The miRNA OneArray® v3).

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