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Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow-Derived Macrophage Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow-Derived Macrophage Transcriptomes

PROVIDER: PRJNA553507 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow-Derived Macrophage Transcriptomes

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during lncRNA-GMR-deficient Bone marrow-derived macrophages and the control cells. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis.Macrophages RNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between lncRNA-GMR-deficient macrophages and the control cells.

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Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow Derived Mast Cell Transcriptomes

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during Tet2-deficient bone marrow derived mast cell (BMMC) and the control cells. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse BMMCs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse IL-3 and SCF. BMMCs were stained to confirm the surface expression of FcɛRI and c-Kit. Cells with purity >97.5% were used for subsequent experiments. BMMCs RNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between Tet2-deficient BMMC and the control cells.

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Sirt3K223R Bone-Marrow derived Macrophages Transcriptomes

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