Project description:The purpose of this experiment is to deep sequence transcripts in WT mice and mice lacking Lingo3, and compare gene expression in the epithelial cells of the colon between wild type and mutant mice. Colon tissue was isolated from each mouse, and epithelial cells were removed from the colon by washing the tissue with 5 mM EDTA + 2.5 mM DTT solution.
Project description:The purpose of this experiment is to deep sequence transcripts in WT mice and mice lacking Lingo2, and compare gene expression in the epithelial cells of the colon between wild type and mutant mice. Colon tissue was isolated from each mouse, and epithelial cells were isolated by EDTA wash
Project description:Provides a set of enriched normal colon epithelial cells to use as a baseline for disease of the colon We present a dependable expression reference data set for non-neoplastic colonic epithelial cells. An enriched population of fresh colon epithelial cells were obtained from non-neoplastic, colectomy specimens and analyzed using Affymetrix GeneChip Exon 1.0 ST arrays. The findings from this study represent the first comprehensive expression profile for non-neoplastic colonic epithelial cells reported. For demonstration purposes, we have compared the data derived from these cells to a publically available set of tumor and normal matched colon data. This analysis allowed an assessment of global gene expression alterations and demonstrated that adjacent normal tissues, with a high degree of cellular heterogeneity, are not always representative of normal cells for comparison to tumors which arise from the colon epithelium. We also examined alternative splicing events in tumors compared to normal colon epithelial cells. Our analysis of splice variants on a genome-wide scale illustrate that this is a very labor intensive procedure, requiring vigilant examination of the data. It is projected that the contribution of this set of data derived from pure colonic epithelial cells will enhance studies in colon-related disease and offer a vital baseline for studies aimed at elucidating the mechanisms of alternative splicing.
Project description:We performed a single-cell transcriptome analysis of proximal colon epithelial cells from 4 Gpr35 KO and 4 WT female littermates mice at a steady state.
Project description:Mucosal healing is a key treatment goal for inflammatory bowel disease, and epithelial regeneration is required for an intact gut epithelium. Long noncoding RNAs are involved in the development of inflammatory bowel disease. Here, we investigated the role of a novel long noncoding RNA, Gm31629, in epithelial regeneration. We used RNA-seq to to detail the global programme of gene expression in distal colon tissues from WT and Gm31629-/-.