Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. The goals of this study are to analysis the different mRNA expression between transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. Quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. We performed mRNA-seq in the NEAT1_2 knockdown group and NC group in the K1 cell line. We found that after knockdown of NEAT1_2, 615 mRNAs were upregulated and 2364 mRNAs were downregulated.
Project description:In order to screen the target genes regulated by lncMGPF,we transfected the siRNA to knock down lncMGPF gene (si-lncMGPF) and negative control siRNA (si-NC), induced cells to differentiate for 2 days.
Project description:We transfected keratinocytes cell line (HaCaT cells) with si-NC and si-eIF4E, and added M5 into the cell culture medium to reveal the function of eIF4E in the in vitro psoriasis cell model.
Project description:Purpose: The purpose is to systemically identify RNA expression changes after lnc-Dpf3 knockdown in CCR7-stimulated mDCs Methods: We profiled RNA seq in mDCs transfected with si-Lnc-Dpf3 or si-NC and stimulated with or without CCR7 ligand CCL19 and CCL21. Results: CCR7 stimulation induced profound RNA expression changes in mDCs, and a set of genes were differentially expressed in lnc-Dpf3-knockdown DC
Project description:We transfected siRNA-EIF4G2 and si-NC into Hep3B cells to explore the differentially expressed genes in cells and the affected intracellular signaling pathways after EIF4G2 knockdown
Project description:RAW264.7 cell was stably transfected with Tim-3 siRNA(SI) or negative control(NC) and were analyzed for microRNA expression Differerently expressed microRNA in Tim-3 knockdown RAW264.7 cells were summarized and were used for further analysis RAW264.7 cells stably transfected with Tim-3 siRNA(SI) or negative control(NC) and were used to collect microRNA without any other treatment
