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Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

ABSTRACT: Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

PROVIDER: PRJNA556421 | ENA |

REPOSITORIES: ENA

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Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

Project description:Overexpression of activin A by keratinocytes accelerates excisional wound healing in mice. Activin-promoted wound healing is mediated via the stroma, specifically by the wound immune cells and fibroblasts. To determine if activin A alters the gene expression profile of wound fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from excisional skin wounds of activin overexpressing mice. We found that activin induces a pro-fibrotic gene expression profile of these fibroblasts, leading to the upregulation of genes involved in collagen biosynthesis and remodeling.

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Analysis of the expression profile of skin fibroblasts FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

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Analysis of the expression profile of skin macrophages FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

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Transcriptome profiling during cutaneous porcine wound repair.

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Transcriptome profiling during cutaneous porcine wound repair.

Project description:The expression profile during wound repair of cutaneous excisional wound (2x2 cm) was studied. The tissue was sampled from the centre of the open wound (day 3/7/14) or centre of the wound scar (day 21/35/70). The microarray slide were scanned in low intensity scan and high intensity scan mode. Wound tissue (day 3, day 7, day 14, day 21, day 35, day 70, n = 4 per interval) vs. control - uninjured skin (day 0, n=4 ). In each interval two biological replicates of four were labeled with flip dyes.

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microRNA expression in skin and wound edge tissue at different time points

Project description:Change in miRNA expression pattern during different phases of wound healing following full thickness excisional wounding was studied.

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microRNA expression in skin and wound edge tissue at different time points

Project description:Change in miRNA expression pattern during different phases of wound healing following full thickness excisional wounding was studied. In the study presented here, a comprehensive profiling of all the mouse/rat miRs annotated in the miRBase 8.0 was performed using the FlexmiRM-bM-^DM-" MicroRNA Labeling Kit

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