Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None. Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes. For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..

| 2612481 | ecrin-mdr-crc

Identification of the molecular basis of anti-cancer effect of Huaier.

Project description:Intervention type:DRUG Name of intervention:Huaier Dose form / Japanese Medical Device Nomenclature:GRANULES Route of administration / Site of application:ORAL Dose per administration:20? g Dosing frequency / Frequency of use:OTHER, SPECIFY 20g? per day Planned duration of intervention:3 months to extending if necessary Intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary detailes of teratment arms:hepatocellular carcinoma, breast cancer, colorectal cancer and related gastrointestinal cancers, urologic cancers including prostate cancer, pancreas cancer, and lung cancer, etc. Comparative intervention name:None Dose form / Japanese Medical Device Nomenclature: Route of administration / Site of application: Dose per administration: Dosing frequency / Frequency of use: Planned duration of intervention: Intended dose regimen: Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes. For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis. Study Design: Comparative test, None, No, open(masking not used), EXPLORATORY

| 98681 | ecrin-mdr-crc

Characterization of a macrophage population under the control of nerves in the gastrointestinal tract

Project description:Primary objectives: Characterization of the macrophage population subset that is modulated by enteric neurons Primary endpoints: Characterization of the macrophage population subset that is modulated by enteric neurons via RNA sequencing

| 2538227 | ecrin-mdr-crc

Mus Musculus, Homo Sapiens

Project description:small RNA sequencing

| PRJNA408072 | ENA

Project description:Small RNA sequencing

| PRJNA647248 | ENA