Project description:TubA is a specific inhibitor of HDAC6, it could regulate oocytes maturation by regulating acetylation of α-tubulin. However, weather it effects maternal mRNA expression remains unkown. Therefore, in this study,we got in vitro maturated MII oocytes in control and 0.1μg/mL treated .Our study suggests that TubA mainly downregulated cell cycle and DNA methylation related genes.
Project description:In order to establish an obese mouse model, female mice were continuously fed with a high-fat diet (HFD) or a normal diet (control) for 16 weeks beginning at three weeks of age. In this paper, these mice are termed ‘HFD mice’ and ‘control mice’, respectively. Accordingly, we call their oocytes ‘HFD oocytes’ and ‘control oocytes’. Substantial evidence indicates that the effects of maternal obesity on embryo/offspring development can be attributed to factors within the oocyte (9). To identify such potential effectors, we performed a comparative proteomic analysis of ovulated MII oocytes from control and HFD mice.
Project description:This dataset consists of germinal vesicle (GV) and metaphase II (MII) oocytes from B6, D2, and B6xD2 F1 mice. Oocytes from a single mother were pooled as one biological replicate to become one sample. The goal of the study was to explore the genetic effects on oocyte quality, and more specifically, to determine a viable explanation for the hybrid vigor phenotype displayed in F1 mice. This study is the first to combine oocytes of different stages and different genotypes (two parental inbred strains and F1 hybrid) into a single analysis capable of identifying molecular characteristics that distinguish F1 hybrids.
Project description:Purpose: To understand how sex chromosome complement, XX, XO and XY, influences the transcriptome in the oocytes of grwoth phase. Methods: Oocytes of 50 and 60 µm in diameter were isolated from mouse ovaries at 18 dpp and subject to RNA-sequencing. Results: (1) Many X-linked genes are subject to X chromosome dosage dependent expression. (2) Many genes are expressed from both short and long arms of the Y chromosome. (3) The transcriptome landscape in XY oocytes is closer to XX oocytes than XO oocytes. (4) About 10 genes are differentially expressed in XY oocytes compared to XX or XO oocytes. Conclusions: The differences in XY oocytes became exacerbated to differ from XX or XO oocytes near the end of growth phase.