Project description:To determine functions of circMbl in brain in vivo we generated genetically encoded shRNA directed against individual circRNA-specific back-spliced junctions. We analyzed gene expression by 3' RNA-seq from control (actin-Gal4) and flies expressing the shRNA under the control of a constitutive driver (actin-Gal4; circRNA KD).

2023-12-01 | GSE163797 | GEO

HEK293 WT and TOM70 KD cell lines expressing HA-tagged Orf9b grown on galactose media

Project description:Comparison of whole cell proteome of HEK293 WT and TOM70 KD cell lines expressing HA-tagged Orf9b grown on galactose media

2023-08-30 | PXD040494 | Pride

HEK293 WT and TOM70 KD cell lines expressing HA-tagged Orf9b grown on glucose media

Project description:Comparison of whole cell proteome of HEK293 WT and TOM70 KD cell lines expressing HA-tagged Orf9b grown on glucose media.

2023-08-30 | PXD040491 | Pride

RNA-Seq analysis of MIN6 cells following control KD or Paupar KD

Project description:Aim:Transcriptional analysis of the MIN6 beta cell line following control or Paupar lncRNA KD to identify genes regulated by Paupar Methods:MIN6 cells subjected to either control or Paupar KD were transfected for 48 hours before extraction of total RNA using the Qiagen Mini kit. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 30 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq2. Results: We did not observe any signficant changes in genes important for beta cell function Conclusion: Paupar does not have a signficant regulatory function in beta cells

2019-10-10 | GSE132072 | GEO

RNA-seq analysis of hES-RPE cells in 14d from LHX2 KD and control - non-specific shRNA

Project description:RNA-seq analysis was applied in duplicates to hES-RPE cells in 14d: LHX2-KD and control - non-specific shRNA

2022-11-21 | GSE178163 | GEO

Control ChIP-seq from HEK293 (ENCSR000EVA)

Project description:Control ChIP-seq on human HEK293 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2019-08-30 | GSE136429 | GEO

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