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Dual-RNA sequencing of dendritic cells challenged with Aspergillus fumigatus

ABSTRACT: Dual-RNA sequencing of dendritic cells challenged with Aspergillus fumigatus

PROVIDER: PRJNA558954 | ENA |

REPOSITORIES: ENA

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Dual-RNA sequencing of dendritic cells challenged with Aspergillus fumigatus

Project description:Dendritic cells and A. fumigatus were co-cultured with two different MOIs (1 or 0,5) for 2, 4 or 6 hours. Single-cultures of dendritic cells and A. fumigatus serve as controls. RNA was isolated and processed for next generation sequencing.

2020-10-01 | GSE135450 | GEO

Triple-Sequencing of dendritic cells coinfected with Aspergillus fumigatus and cytomegalovirus

Project description:Human dendritic cells were coinfected with Aspergillus fumigatus and human cytomegalovirus. Single-cultures or single-infections served as controls. RNA was isolated and processed for next generation sequencing.

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Gene expression profiles of human immature dendritic cells after 3h, 6h and 12h of co-cultivation with Aspergillus fumigatus

Project description:Gene expression profiles of human immature dendritic cells after 3h, 6h, 9h and 12h of co-cultivation with Aspergillus fumigatus were compared to expression profiles from human immature dendritic cells after 3h, 6h, 9h and 12h of cultivation. We used microarrays to detail the gene expression of human immature dendritic cells after 3h, 6h, 9h and 12h of co-cultivation with Aspergillus fumigatus

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Comparative genomic hybridization of Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae and Neosartorya fischeri with Aspergillus fumigatus Af293

Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.

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Gene expression profiles of human immature dendritic cells after 3h, 6h and 12h of co-cultivation with Aspergillus fumigatus

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The In Vitro Interaction of Aspergillus fumigatus and Human immature Dendritic Cells

Project description:Dendritic cells (DC) play an important role in host immunity by acting as a bridge between the innate and adaptive immune systems. They are antigen presenting cells that obtain microbial antigens by direct phagocytosis of the microbe or by cross presentation of antigens taken up from the surrounding environment. Monocyte derived DC were co-cultured with resting conidia of Aspergillus fumigatus at an MOI of 5 for 12 hours, cells were sampled every three hours. RNA was extracted from both organisms at each time point and hybridised to micro-arrays, whole genome Aspergillus fumigatus array (JCVI) and a custom immune array for DC. The genes up-regulated by DC in the presence of A. fumigatus indicated that the cells were producing a pro-inflammatory response. There was an increase in IL8 expression over time confirming its association with germ tube emergence. Over the course of the experiment there was increased expression of 210 genes by A. fumigatus, GO analysis indicated significant up-regulation of the following biological processes: fermentation, drug transport, pathogenesis, transport, tyrosine catabolism and response to oxidative stress. There were two clusters of temporally regulated genes showing up regulation before 6hr and after 6 hrs. This may be related to the increased mortality exhibited in DC at 6h. The initial analysis of A. fumigatus gene expression in response to DC shows similarity to its response to neutrophils with an up-regulation in catabolism and response to oxidative stress. A. fumigatus AF293 cells were grown in the presence and absence of human dendritic cells for 0h - 12h. Hybridizations were performed with biological replicates and flip-dye pairs.

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Project description:This SuperSeries is composed of the following subset Series: GSE21353: Gene expression profiles of human immature dendritic cells after 3h, 6h and 12h of co-cultivation with Aspergillus fumigatus GSE28806: The temporal dynamics of differential gene expression in human alveolar epithelial and endothelial cells interacting with the human pathogenic mould Aspergillus fumigatus in vitro Refer to individual Series

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The In Vitro Interaction of Aspergillus fumigatus and Human immature Dendritic Cells

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Effect of amphotericin B on Aspergillus fumigatus

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