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Identification of differentially-expressed piRNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

ABSTRACT: Identification of differentially-expressed piRNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

PROVIDER: PRJNA559373 | ENA |

REPOSITORIES: ENA

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Identification of differentially-expressed piRNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

Project description:The aim of this study was to identify non-coding RNA that were differentially expressed 7 days after induction of osteogenic or chondrogenic differentiation in human bone marrow-mesenchymal stromal cells (MSCs).

2020-02-18 | GSE135587 | GEO

Identification of differentially-expressed miRNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

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Identification of differentially-expressed circular RNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

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Identification of differentially-expressed miRNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

Project description:Identification of differentially-expressed miRNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

| PRJNA559368 | ENA

Identification of differentially-expressed circular RNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

Project description:Identification of differentially-expressed circular RNAs in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

| PRJNA560401 | ENA

Transcription profiling of human bone marrow mesenchymal stromal cells before and after stimulation with dexamethasone to study the early molecular processes of osteogenic differentiation

Project description:Human adult mesenchymal stromal cells (hMSC) have the potential to differentiate into chondrogenic, adipogenic or osteogenic lineages, providing a potential source for tissue regeneration. An important issue for efficient bone regeneration is to identify factors that can be targeted to promote the osteogenic potential of hMSCs. Using transcriptomic analysis, we found that integrin alpha5 (ITGA5) expression is upregulated during dexamethasone-induced hMSCs osteoblast differentiation. Gain-of-function studies showed that ITGA5 promotes the expression of osteoblast phenotypic markers as well as in vitro osteogenesis in hMSCs. Downregulation of endogenous ITGA5 using shRNA blunted osteoblast marker expression and osteogenic differentiation. Pharmacological and molecular analyses showed that the enhanced hMSCs osteoblast differentiation induced by ITGA5 was mediated by activation of FAK/ERK1/2-MAPKs and PI3K signaling pathways. Remarkably, activation of ITGA5 using a specific antibody that primes the integrin or a peptide that specifically activates ITGA5 was sufficient to enhance ERK1/2-MAPKs and PI3K signaling and to promote osteoblast differentiation and osteogenic capacity of hMSCs. We also demonstrate that hMSCs engineered to over-express ITGA5 exhibited a marked increase in their osteogenic potential in vivo. These findings not only reveal that ITGA5 is required for osteoblast differentiation of adult human MSCs but also provide a novel targeted strategy using ITGA5 agonists to promote the osteogenic capacity of hMSCs, which may be used for tissue regeneration in bone disorders where the recruitment or capacity of MSCs is compromised. Experiment Overall Design: Gene expression profiles were generated from bone marrow MSC before and 1, 3 and 7 days after stimulation with 10E-7M dexamethasone to study the early molecular processes of osteogenic differentiation. 3 replicates per timepoint.

2009-09-18 | E-GEOD-18043 | biostudies-arrayexpress

Identification of differentially-expressed miRNA, piRNA and circRNA in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

Project description:Identification of differentially-expressed miRNA, piRNA and circRNA in early osteogenic and chondrogenic differentiation of human bone marrow-mesenchymal stromal cells (hMSCs)

| PRJNA559367 | ENA

Osteogenic differentiation of human mesenchymal stem cells (hMSCs) within a perfusion bioreactor system

Project description:hMSCs were cultured for eight days within perfused three-dimensional matrices under conditions of oscillatory, continuous or static fluid flow of osteogenic media.

2009-09-16 | GSE18114 | GEO

Identification of differentially-expressed piRNAs after human bone marrow-mesenchymal stromal cells (hMSCs) detachment

Project description:Identification of differentially-expressed piRNAs after human bone marrow-mesenchymal stromal cells (hMSCs) detachment

| PRJNA530998 | ENA

Effect of electromagnetic fields on the chondrogenic differentiation under microgravity conditions

Project description:A combination therapy of electromagnetic fields (EMF) and simulated microgravity (SMG) has not been examined in regenerative medicine of cartilage. In the present study, a bioreactor system using extremely low-frequency EMF and SMG was applied during the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). It was hypothesized that a beneficial effect of EMF regarding chondrogenesis (COL2A) could be combined with an avoiding effect of SMG regarding hypertrophy (COLXA1) of cartilage. Pellet cultures of hMSCs formed cartilaginous tissue under the addition of growth factors (FGF; TGF-Î²3). Pure SMG reduced COLXA1 expression but also COL2A expression of hMSCs. Pure EMF showed no gene expression changes of hMSCs during chondrogenic differentiation. Combining EMF/SMG resulted in a re-increase of COL2A but did not reach control levels. The COL2A to COLXA1 ratio of combined EMF/SMG was not significantly different from control levels. The combination therapy of EMF/SMG did not significantly improve the chondrogenic potential of hMSCs. chondrogenic differentiation, electromagnetic stimulation-control, 1 timepoint with/without stimulation

2015-12-31 | E-GEOD-57298 | biostudies-arrayexpress

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