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Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

ABSTRACT: Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

PROVIDER: PRJNA560773 | ENA |

REPOSITORIES: ENA

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Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

Project description:The cellular source for hepatocyte transplantation and liver regeneration in chronically hepatic diseases is a fundamental topic in liver biology. A successful therapeutic approach based on a benificial stem/progenitor cells would involve replacing damaged cells or restoring homeostasis to the areas that underlie the fibrotic response. This study aims to isolate and culture hepatocyte and non-parenchymal cells derived liver progenitor-like cells (HepLPCs and NPC-LPCs) to identify a beneficial cell sources for cell-based therapy against chronic liver injury. Two types of lineage tracing models including tdTomato mice with AAV8-TBG-Cre and AlbCreERT/R26GFP were purified for hepatocytes and non-parenchymal cells respectively, and cultured for transition to progenitor-like cells (LPCs) in TEM. The differences between them were clarified by RNA sequencing, suggesting that HepLPCs retaining basal levels of hepatic function and transcription factors (TFs), including Foxa3. Organoids and 3D hepatic spheroids culturing systems were used for further analysis of bile duct and hepatic functioning diversity. When transplanting in mice with CCL4 treatment, HepLPCs displayed the characteristics of TFs regulatory reactivation and physiological balance restoration, resulting in a significant improvement in liver fibrosis compared with NPC-LPCs, which are crucial for liver regeneration in the treatment of patients with a broad range of hepatic fibrosis.

2020-07-30 | GSE135951 | GEO

TAK1 deficient liver parenchymal cells with Rip3 double knockout TAK1LPC

Project description:Genomic copy number profiling of TAK1 deficient liver parenchymal cells with Rip3 double knockout Sample vs. Reference (pool of 4 normal liver tissues)

2013-05-01 | E-GEOD-45206 | biostudies-arrayexpress

Single-cell RNA sequencing of liver parenchymal cells from murine model of endotoxemia

Project description:Liver has a crucial role in the regulation of immune defense in systemic infections. During endotoxemia, the liver transits from an immune-tolerant towards an immune-active state and forms the first line of defense against invading microorganisms. the role of liver parenchymal cells in endotoxemia remains unintelligible. To characterize the liver parenchymal cells in endotoxemia liver, we performed single-cell RNA sequencing of liver parenchymal cells from healthy C57BL/6J mice and murine model of endotoxemia at early or late stage. The single-cell RNA-seq analyses revealed the heterogeneity of liver parenchymal cells in endotoxemia liver.

2022-04-06 | GSE186554 | GEO

TAK1 deficient liver parenchymal cells with Rip3 double knockout TAK1LPC

Project description:Genomic copy number profiling of TAK1 deficient liver parenchymal cells with Rip3 double knockout

2013-05-01 | GSE45206 | GEO

Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages

Project description:Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages

| PRJEB39325 | ENA

Smart-seq2 single-cell RNA sequencing of mouse liver non-parenchymal cells

Project description:Smart-seq2 scRNA-seq of liver non-parenchymal cells from 3 high-fat diet and 3 control mice.

2023-04-29 | GSE230440 | GEO

In vitro activation and reversion of human primary hepatic stellate cells

Project description:Liver fibrosis is characterized by the excessive formation and accumulation of matrix proteins as a result of wound healing in the liver. A main event during fibrogenesis is the activation of the liver resident quiescent hepatic stellate cell (qHSC). Recent studies suggest that reversion of the activated HSC (aHSC) phenotype into a quiescent-like phenotype could be a major cellular mechanism underlying fibrosis regression in the liver, thereby offering new therapeutic perspectives for the treatment of liver fibrosis. The goal of the present study is to identify experimental conditions that can revert the activated status of human HSCs and to map the molecular events associated with this phenotype reversion by gene expression profiling Transcriptomic profiling of primary human quiescent HSCs (qHSCs), in vitro activated HSCs (aHSCs) and in vitro reverted HSCs (rHSCs). The cell types come from different donors (This is detailed in the original manuscript.).

2015-08-12 | E-GEOD-68001 | biostudies-arrayexpress

A Single-Cell Atlas of Murine Liver Non-parenchymal Cells in Typical Disease Models

Project description:We provided a comprehensive single-cell transcriptomic atlas of murine liver non-parenchymal cells (NPCs) in different diseases and analyzed the heterogeneity of inter- and intra-group NPCs. Our study offers a valuable resource for better understanding liver physiology and pathology, and bring new directions for the development of clinical diagnostic markers and effective therapeutic targets.

2021-08-01 | GSE166178 | GEO

In vitro activation and reversion of human primary hepatic stellate cells

2015-08-12 | GSE68001 | GEO

Human liver cells proteome

Project description:The human liver functions through a complex interplay of parenchymal and non-parenchymal cells, which participate in performing many essential aspects of metabolism and secretion. Mass spectrometry-based proteomic analysis of intact tissue has improved the understanding of these processes, by providing an in-depth view of the human liver proteome. However, the predominance of parenchymal cells, i.e. hepatocytes, means that the total tissue proteome mainly reflects hepatocyte expression. In this study, we therefore used quantitative label-free proteomic analysis to analyze the proteomes of the major parenchymal and non-parenchymal cell types in the human liver, i.e. hepatocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatic stellate cells. We found that our data recapitulated specific functional differences between cell types, meaning that it can be used to reliably probe various aspects of cellular interplay. Thus, the data we provide constitutes a unique resource for exploring the human liver proteome at major cell type resolution.

2020-04-09 | PXD012615 | Pride

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