Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiles of Streptomyces coelicolor A3(2) wild type and wblC (whiB7) mutant treated with tetracycline

ABSTRACT: Transcriptome profiles of Streptomyces coelicolor A3(2) wild type and wblC (whiB7) mutant treated with tetracycline

PROVIDER: PRJNA561423 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome profiles of Streptomyces coelicolor A3(2) wild type and wblC (whiB7) mutant treated with tetracycline

Project description:We determined Streptomyces coelicolor genes that are directly regulated by WblC (or WhiB7), an actinobacterial transcription factor that activates expression of intrinsic resistance in response to translation-inhibitory antibiotic stress. Identification of differentially expressed genes in wblC mutant by RNA-seq and WblC binding sites in wild type by ChIP-seq identified more than 300 genes as WblC regulon. This series encompasses the RNA-seq data of our study.

2020-03-28 | GSE136167 | GEO

Streptomyces coelicolor A3(2) Timecourse Proteomics

Project description:Streptomyces coelicolor A3(2) shotgun proteomics. S. coelicolor A3(2) was fermented in GYM.

2022-03-23 | MSV000089111 | MassIVE

Comparative genomic hybridization Streptomyces coelicolor A3(2) strains

Project description:The chromosomes of several widely used laboratory derivatives of Streptomyces coelicolor A3(2) were found to have 1.06 Mb inverted repeat sequences at their termini (i.e. long-terminal inverted repeats; L-TIRs), which are 50 times the length of the 22 kb TIRs of the sequenced S. coelicolor strain M145. The L-TIRs include 1005 annotated genes and increase the overall chromosome size to 9.7 Mb. The 1.06 Mb L-TIRs are the longest reported thus far for an actinomycete, and are proposed to represent the chromosomal state of the original soil isolate of S. coelicolor A3(2). S. coelicolor A3(2), M600 and J1501 possess L-TIRs, whereas approximately half the examined early mutants of A3(2) generated by ultraviolet (UV) or X-ray mutagenesis have truncated their TIRs to the 22 kb length. UV radiation was found to stimulate L-TIR truncation. Two copies of a transposase gene (SCO0020) flank 1.04 Mb of DNA in the right L-TIR, and recombination between them appears to generate strains containing short TIRs. This TIR reduction mechanism may represent a general strategy by which transposable elements can modulate the structure of chromosome ends. The presence of L-TIRs in certain S. coelicolor strains represents a major chromosomal alteration in strains previously thought to be genetically similar.

2005-07-21 | E-SMDB-2740 | biostudies-arrayexpress

Genome plasticity in Streptomyces: identification of 1 Mb TIRs in the S. coelicolor A3(2) chromosome

2005-07-26 | GSE2994 | GEO

Transcriptome profiles of Streptomyces coelicolor A3(2) wild type treated with kanamycin and iron

Project description:We determine genes that responsible for iron induced resistance to kanamycin in Streptomyces coelicolor. Iron acts as a inducing agent for resistance to bactericidal antibiotics with concentration dependent manner. Identification of iron-dependent differentially expressed genes in wild-type by RNA-seq identified more than 100 genes. This series encompasses the RNA-seq data of our study.

2022-03-10 | GSE168567 | GEO

Genome-wide identification of WblC (WhiB7) binding sites in Streptomyces coelicolor A3(2) treated with tetracycline

Project description:Genome-wide identification of WblC (WhiB7) binding sites in Streptomyces coelicolor A3(2) treated with tetracycline

| PRJNA561422 | ENA

Streptomyces coelicolor A3(2)

Project description:Streptomyces coelicolor A3(2) SigR regulon

| PRJNA148535 | ENA

Genome-wide identification of WblC (WhiB7) binding sites in Streptomyces coelicolor A3(2) treated with tetracycline

2020-03-28 | GSE136166 | GEO

WblC- and tetracycline-dependent shifts in the composition of Streptomyces coelicolor ribosomal proteome

Project description:WblC, also known as WhiB7, is a widely conserved WhiB-like transcription factor in actinomycetes that activates transcription of many targets upon antibiotic challenge to bring about intrinsic resistance to a wide range of translation-targeting antibiotics. As we found that WblC controls many genes involved in translation and that WblC promotes translation rate upon antibiotic stress in the model actinomycetes Streptomyces coelicolor, we speculated that WblC might alter the protein composition of ribosome during antibiotic stress. To test this, we prepared 70S ribosome fraction from wild-type S. coelicolor cells untreated or treated with tetracycline and ΔwblC mutant treated with tetracycline, and then compared the protein compositions of each 70S samples by mass spectrometric quantification.

2020-05-27 | PXD015538 | Pride

Genome plasticity in Streptomyces: identification of 1 Mb TIRs in the S. coelicolor A3(2) chromosome

2005-07-26 | E-GEOD-2994 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data