Project description:Dendritic cells were infected with human cytomegalovirus (CMV) for 4, 8 or 12 hours. Single-cultures of dendritic cells serve as controls. RNA was isolated and processed for next generation sequencing.
Project description:Analysis of dendritic cells (DCs) at 6h and 48h after infection with cytomegalovirus (CMV) versus non infected. CMV tends to induce immunosuppression followed by lasting immunity. DCs appear to play a role in this effect. Results provide insights into CMV/DC interactions and suggest mechanisms for CMV-regulated early immune response. Keywords: gene expression array-based, count In the present study, immature monocyte-derived dendritic cells were generated from CMV neg healthy blood donors and infected or not with an endotheliotropic CMV strain. Gene expression modulation by the CMV infection was studied in a time course assay (non-infected - NI - vs 6h or 48h post-infection. Patient C has no 48h sample
Project description:Analysis of dendritic cells (DCs) at 6h and 48h after infection with cytomegalovirus (CMV) versus non infected. CMV tends to induce immunosuppression followed by lasting immunity. DCs appear to play a role in this effect. Results provide insights into CMV/DC interactions and suggest mechanisms for CMV-regulated early immune response. Keywords: gene expression array-based, count
Project description:Human dendritic cells were coinfected with Aspergillus fumigatus and human cytomegalovirus. Single-cultures or single-infections served as controls. RNA was isolated and processed for next generation sequencing.
Project description:We report the transcriptome profiles of guinea pig cytomegalovirus (Caviid betaherpesvirus 2 ; GPCMV) infected and uninfected cells. RNA was harvested at an early time post-infection. Data sets from a lung fibroblast cell line (JH4), primary amnion derived cells (1° AECs), and HPV16 E6/E7-transduced amniotic epithelial cells (AECD) is shared.
Project description:Previous articles have reported that mouse cell lines infected with hepatitis virus do not produce interferon, which only causes natural immune responses when infected with macrophages and dendritic cells. Therefore, we collected the cells of MHV-infected mouse dendritic cells for 18 hours, and detected the immune-related factors and other altered genes in the cells after infection by Proteomics analysis