Project description:Purpose: Cholesterol is an essential nutrient for diverse biological processes in all organisms. However, the role of cholesterol in immune function remains understudied. Hence the goal is to obtain cholesterol-mediated immune genes Methods: Hermaphrodite C. elegans (var. Bristol) wild type (N2) were grown on 20 along side with control, which is 5µg/ml cholesterol and harvested at L4 stage. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analyses of the transcriptomic data show that cholesterol-mediated immune gene that could enhance the fight against infectious diseases Conclusions: This study showed a novel role for cholesterol in the enhancement of the innate
Project description:Purpose: Cholesterol is an essential nutrient for diverse biological processes in all organisms. However, the role of cholesterol in immune function remains understudied. Hence the goal is to obtain cholesterol-mediated immune genes Methods: Hermaphrodite C. elegans (var. Bristol) wild type (N2) were grown on 0µg/ml along side with control, which is 5µg/ml cholesterol and harvested at L4 stage. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analyses of the transcriptomic data show that cholesterol-mediated immune gene that could enhance the fight against infectious diseases Conclusions: This study showed a novel role for cholesterol in the enhancement of the innate
Project description:Investigation of whole genome gene expression level changes in C. elegans eat-2, acs-20 and eat-2; acs-20 mutant compared to the wild-type N2 strain.
Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 rsd-2 and Bristol N2 rsd-6 single mutants, compared to late-generation strains at 25°C and 20°C
Project description:Transcriptional profiling of N2 (WT) and miR-85(m4117) Caenorhabditis elegans at larval stage 4 (L4) compared at either control temperature (20°C) or after 3hr HS (35°C).
Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing culture treated with 20 µg/mL tannic acid against non-treated culture grown in Mueller-Hinton media