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Microarray profiling of in vitro expanded FRCs cultured alone or with activated T cells

ABSTRACT: Microarray profiling of in vitro expanded FRCs cultured alone or with activated T cells

PROVIDER: PRJNA564111 | ENA |

REPOSITORIES: ENA

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Microarray profiling of in vitro expanded FRCs cultured alone or with activated T cells

Project description:To investigate whether FRCs express molecules capable of promoting the functions of activated T cells, we expanded FRCs from primary lymph node stromal cell (LNSC) cultures as previously described (Lukacs-Kornek et al., Nature Immunology, 2011), and then cultured them alone or with splenocytes activated with soluble antibody (0.25μg/ml) against CD3 (anti-CD3) and anti-CD28 for 16 hours. FRCs co-cultured with activated T cells upregulated expression of genes encoding molecules known to dampen T cell function such as Arg1, CD274 and Nos2. However, in response to activated T cells, FRCs also upregulated molecules with immunostimulatory capabilities such as Icosl, Cd40 and Il6.

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Small RNA in endothelial cells and EVs cultured alone or with tumor cells

Project description:RNA was harvested from endothelial cells cultured alone or with tumor cells and the associated endothelial extracellular vesicles.

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Long RNA in endothelial cells and EVs cultured alone or with tumor cells

Project description:RNA was harvested from endothelial cells cultured alone or with tumor cells and the associated endothelial extracellular vesicles.

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Comparison of the DNA methylation of in vitro cultured bovine SAM-treated blastocysts and non-treated blastocysts

Project description:Epigenetics (DNA methylation) profiling of bovine in vitro cultured expanded blastocysts (EB) comparing control non-treated expanded blastocysts with SAM-treated expanded blastocysts. S-Adenosyl methionine (SAM) is the global methyl donor providing methyl

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mRNA and lncRNA microarray of vCAF modulating intrahepatic cholangiocarcinoma progression

Project description:We found that the isolated and expanded MCAM positive vCAF subpopulations significantly accelerated ICC growth. To further investigate the effect of vCAF on ICC cells (GFP-FRH or GFP-RBE), we co-cultured the vCAF with GFP ICC cells or ICC cells alone for seven days, then we sorted the GFP-FRH or GFP-RBE cells via FACS and conducted transcriptome microarray analysis of GFP ICC cells alone or vCAF co-cultured GFP-ICC cells.

2020-06-06 | GSE148773 | GEO

RNA sequencing of B-cell acute lymphoblastic leukemia-derived mesenchymal stem cells cultured alone and co-culture with leukemia cells

Project description:To explore the possible differences in gene expression profiles between MSCs cultured alone and MSCs in leukemia niches

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Transcription profiling of human neutrophils cultured alone or with opsonized Candida albicans

Project description:Neutrophils were separated from human blood. Then they were either cultured alone or with opsonized Candida albicans (strain CAF2-1) for 2 hours in RPMI1640 at 37 deg C.

2007-05-31 | E-MEXP-914 | biostudies-arrayexpress

Small RNA in endothelial cells and EVs cultured alone or with tumor cells

Project description:Small RNA in endothelial cells and EVs cultured alone or with tumor cells

| PRJNA705191 | ENA

Long RNA in endothelial cells and EVs cultured alone or with tumor cells

Project description:Long RNA in endothelial cells and EVs cultured alone or with tumor cells

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Microarray evaluation of in vitro cultured Sox9+ mouse lung progenitors isolated from E12.5 embryonic lung progenitors

Project description:The study aims to show that Sox9+ Chd1+ mouse embryonic lung progenitors can be isolated and expanded long-term in 3D culture while maintaining their multipotency. in vitro cultured Sox9+ Chd1+ lung progenitors transcriptionally resemble their in vivo counterparts and show significant difference from adult lung epithelial (Cdh1+ and EpCAM+) and non-epithelial (Cdh1- and EpCAM-) cells

2017-11-01 | E-MTAB-6086 | biostudies-arrayexpress

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