Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphoribotrap-based profiling of activated hypothalamic cells during acute high-fat diet (HFD)-feeding

ABSTRACT: Phosphoribotrap-based profiling of activated hypothalamic cells during acute high-fat diet (HFD)-feeding

PROVIDER: PRJNA566422 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Phosphoribotrap-based profiling of activated hypothalamic cells during acute high-fat diet (HFD)-feeding

Project description:To identify novel neurocircuits activated upon short-term HFD feeding, we employed phosphoribotrap-profiling, which allows for the unbiased identification of alterations in neuronal activation via immunoprecipitation of phosphorylated S6 ribosomal protein-tagged ribosomes from hypothalamic extracts of mice exposed to either 3 days of normal chow diet or HFD-feeding. We have analyzed mRNA selectively expressed in hypothalamic cells activated by a either normal chow diet (NCD)-feeding for 3 days or high fat diet (HFD)-feeding for 3 days. 10-week-old male C57/BL6N mice were put either on a normal chow diet or a high fat diet for 3 days. Afterwards, mice were sacrificed by cervical dislocation. The hypothalamus was rapidly dissected using a stainless steel brain matrix and immediately frozen in liquid nitrogen. Hypothalamic tissues were pooled (8 per IP).

2020-04-16 | GSE137737 | GEO

Hypothalamic astrocytes TRAP-seq in ORX/HFD mice

Project description:3' mRNA-sequencing of the immunoprecipitated and supernatant fractions after Translating Ribosome Affinity Purification (TRAP) targeted to hypothalamic astrocytes RNA (from Aldh1l1eGFP-L10a mice) for 3 experimental conditions: chow diet; high-fat diet; high-fat diet + orchiectomy (Sham-chow n=7, Sham-HFD n=6, ORX-HFD n=7)

2023-04-03 | GSE214683 | GEO

Obesity Remodels Activity and Transcriptional State of a Lateral Hypothalamic Brake on Feeding

Project description:We demonstrate that high fat diet (HFD)-induced obesity alters the transcriptional state of lateral hypothalamic glutamate neurons in mice.

2019-05-02 | GSE130597 | GEO

Genome-wide analysis of gene expression in adipose tissue of female mice after continuous high fat diet feeding

Project description:Analysis of gene expression in adipose tissue of female mice after continuous high fat diet (HFD) feeding for three generations. The hypothesis in this study is that continuous HFD feeding has transgenerational amplification effects to the offspring. Results provide important information on the impacts of over-nutrition over one generation on the offspring, such as transgenerational up-regulated or down-regulated genes. Total RNA was obtained from adipose tissue of the HFD fed mice, including F0, F1 and F2 generations. Normal chow fed mice were used as controls.

2013-05-10 | E-GEOD-37238 | biostudies-arrayexpress

Mouse muscle mitochondrial proteome changes in high fat diet vs. low fat diet

Project description:We investigated remodeling of the mitochondrial proteome to determine mechanisms of changes to lipid oxidation following high-fat feeding. C57BL/6J mice consumed either a high-fat diet (HFD, 60% fat) or low fat diet (LFD, 10% fat) for 12 weeks. Mice were fasted 4 hours then anaesthetized by sodium pentobarbital for tissue collection. A mitochondrial-enriched fraction was prepared from gastrocnemius muscles and underwent proteomic analysis by high-resolution mass spectrometry.

2018-04-12 | MSV000082272 | MassIVE

Genome-wide analysis of gene expression in adipose tissue of female mice after continuous high fat diet feeding

2013-05-10 | GSE37238 | GEO

Quantitative proteomic analysis of oocytes from high-fat diet (HFD)-fed and normal diet mice

Project description:In order to establish an obese mouse model, female mice were continuously fed with a high-fat diet (HFD) or a normal diet (control) for 16 weeks beginning at three weeks of age. In this paper, these mice are termed ‘HFD mice’ and ‘control mice’, respectively. Accordingly, we call their oocytes ‘HFD oocytes’ and ‘control oocytes’. Substantial evidence indicates that the effects of maternal obesity on embryo/offspring development can be attributed to factors within the oocyte (9). To identify such potential effectors, we performed a comparative proteomic analysis of ovulated MII oocytes from control and HFD mice.

2018-05-24 | PXD006641 | Pride

Proteomics of liver from mice fed high fat diet (HFD) or regular chow diet

Project description:Proteomics of liver tissue from mice fed a high fat diet (HFD) or regular chow diet. Data accompany our paper entitled “Dynamic Regulation of N6,2′-O-dimethyladenosine (m6Am) in Obesity” scheduled for publication in Nature Communications, 2021

2021-10-27 | PXD029280 | Pride

Transcriptomic alteration of bone-related cells in aged mice after long-term high fat diet (HFD) feeding

Project description:Aging is associated with osteoporosis and remodeling of bone marrow microenvironment, skewing hematopoiesis. Obesity may accelerate aging process. We use bulk-RNA sequencing to measure the transcriptomic changes of bone cells caused by long-term HFD feeding in aged mice, which mainly includes osteocytes and other bone marrow stromal cells.

2023-11-15 | GSE218105 | GEO

Diet induced obesity is independent of metabolic endotoxemia, Tlr4 signalling and hypothalamic inflammation

Project description:A number of studies have proposed that excess food intake, particularly of high fat diets arise due dysregulation of homeostatic mechanisms regulating neuroendocrine control of appetite and energy balance. Current dogma suggests high fat diets invoke hypothalamic inflammation which reduces hypothalamic sensitivity to metabolic and hormonal cues of conveying peripheral status of energy balance, such as leptin and insulin. A hypothesis for the mechanism leading to hypothalamic inflammation is based on high fat diet mediated changes in gut microbiota which are then proposed to increase circulating levels of lipopolysaccharide (LPS). This in turn activates a hypothalamic inflammatory response via the toll-like receptor (TLR4) and CD14. The aim of this study was to determine hypothalamic gene expression in response to long term feeding of a high fat diet, taking into account the importance of using a control diet with a similar composition and balanced for sucrose content.

2018-10-23 | E-MTAB-6929 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data