Project description:The mechanisms of cardiotoxicity of the three widespread model polycyclic aromatic hydrocarbons (PAHs) retene, pyrene and phenanthrene were explored in rainbow trout (Oncorhyncus mykiss) early life stages. Newly hatched larvae were exposed to sublethal doses of each individual PAH causing no detectable morphometric alterations. Changes in the cardiac proteome were assessed after 7 or 14 days of exposure to each PAH.
Project description:The goals of this study are to obtain the transcriptome profiling (RNA-seq) of different developmental stages and tissues in Ostrinia furnacalis. Methods: O. furnacalis embryos were collected less than 12 h post oviposition. 1st, 3rd and 5th instar of the Asian corn borer, which represents the newly hatched larvae, the middle stage of larvae and the mature larvae, was harvested for subsequent experiments. Pupae and adults were grouped into females and males, then female and male samples were mixed at the ratio of 1:1. The 3rd instar larvae were anesthetized on an ice plate for tissue extraction. The midgut, fat body and silk gland were isolated. All these samples were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: We obtained the transcriptome profiling (RNA-seq) of different developmental stages and tissues in Ostrinia furnacalis Conclusions: Our study representsa detailed analysis of different developmental stages and tissues in Ostrinia furnacalis
Project description:The first period post-hatch with all its stimuli could potentially be stressful for the chicks, and by that affect the welfare of the chicken as they mature. By measuring changes in the transcriptome, along with behavioural phenotypes and hormonal levels, the aim is to investigate short and long term effects on the chicken. One batch of chicken were hatched in a production environment, and underwent the same treatment all newly hatched production animals do (sorting, sexing and transportation). Eggs of the same age were simultaneously incubated and hatched in a control environment in a lab. From the end of day one after hatch, both groups were reared under the same conditions in the lab.
Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on global gene expression using microarray transcriptional profiling in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.
