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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_2

ABSTRACT: NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_2

PROVIDER: PRJNA575183 | ENA |

REPOSITORIES: ENA

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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_4

Project description:NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_4

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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation

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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_1

Project description:We report the genome wide occupancy of p65, H3K4Me3, H3K27Ac and p300 in MCF7-NAIL-WT and MCF7-NAIL-ΔNFκB cells stimulated with TNF alpha for 0min, 45min and 90 min.

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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_2

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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation

Project description:This SuperSeries is composed of the SubSeries listed below.

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NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NFκB mediated chromatin remodelling in inflammation_4

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Project description: Not available

| S-EPMC8458091 | biostudies-literature

Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling

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Expression analysis of nail matrix in mouse.

Project description:Mammalian digit-tip can regenerate upon amputation1-3, like amphibians. It is unknown why this capacity is limited to the area associated with the nail3-5. Here, we show that nail stem cells (NSCs) reside in the Wnt-suppressed proximal nail matrix and that the mechanisms governing NSC differentiation are directly coupled with their ability of orchestrating digit regeneration. Early nail progenitors located distal to the NCS region undergo Wnt-dependent differentiation into nail. Upon amputation, this Wnt activation is required for nail regeneration and also for attracting nerves that promote mesenchymal blastema growth, leading to regeneration of the entire digit. Amputations proximal to the Wnt-active nail progenitors result in failure to regenerate nail/digit. Nevertheless, Î²-catenin stabilization in the NSC region induced their regeneration. These results establish a link between NCS differentiation and digit regeneration, suggesting a utility of the NSCs in developing novel treatments for amputees. Nail matrix cells were harvested from proximal and distal matrix region by microdissection and processed to RNA extraction and hybridization on Affymetrix microarrays. We analyzed two proximal and two distal matrix cells.

2013-03-27 | E-GEOD-45494 | biostudies-arrayexpress

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