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Sequencing of commercially-available pancreatic cancer cell lines reveals selective CYP3A5 overexpression among xenobiotic-metabolizing CYPs.

ABSTRACT: Sequencing of commercially-available pancreatic cancer cell lines reveals selective CYP3A5 overexpression among xenobiotic-metabolizing CYPs.

PROVIDER: PRJNA575858 | ENA |

REPOSITORIES: ENA

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Sequencing of commercially-available pancreatic cancer cell lines reveals selective CYP3A5 overexpression among xenobiotic-metabolizing CYPs.

Project description:Purpose: To know the expression of all cytochrome P450 (CYP) enzymes and related drug metabolizing enzymes in pancreatic cancer models Methods: We obtained expression profiles of 8 commercially-available pancreatic cancer cell lines and one non-cancerous immortalized cell line by RNA-sequencing. The pancreatic cancer cells were compared among themselves and to the non-cancerous control cells. Results: We confirmed reports that CYP3A5 is highly overexpressed in pancreatic cancer models, and additionally report that known xenobiotic-metabolizing CYPs are lowly expressed in comparison.

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Coordinated Changes in Xenobiotic Metabolizing Enzyme Gene Expression in Aging Male Rats: Brown Norway and F344

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Evaluation of commercially available small RNA methods for plant samples

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Transcription profiling of human brain total RNA vs Universal Human Reference RNA on 4 different commercially available microarrays

Project description:Commercially available human genomic microarrays from four different manufacturers were used to compare Human Brain Total RNA against Universal Human Reference RNA (both commercially available) prepared at two different starting amounts (20 µg or 1µg). For each level of RNA, 6 replicates were performed with Human Brain Total RNA labelled with Cy3, and Universal Human Reference RNA labelled with Cy5. The labelling was then reversed (dye flip) creating another 6 replicates. This meant that for each of the four manufacturers there were a total of 24 arrays. Image processing was performed with two different software packages, and data was normalized with three different strategies.

2013-01-30 | E-LGCL-2 | biostudies-arrayexpress

Transcription profiling of human brain total RNA vs Universal Human Reference RNA on 4 different commercially available microarrays

2004-04-01 | E-LGCL-4 | biostudies-arrayexpress

Transcription profiling of male rats Brown Norway and F344 reveals coordinated changes in xenobiotic metabolizing enzyme gene expression in aging

Project description:In order to gain insight into the effects of aging on susceptibility to environmental toxins, we characterized the expression of xenobiotic metabolizing enzymes (XMEs) from the livers of male Brown Norway and F344 rats across the adult lifespan. To examine metabolic processes across lifespan after challenge with a xenobiotic compound, Brown Norway rats were exposed to 1.0 g/kg body weight toluene by oral gavage in corn oil (4ml/kg body weight) or corn oil alone. Experiment Overall Design: Brown Norway rats: Analysis of gene expression profiles for XMEs in male Brown Norway rats 4, 12, and 24 months old. Rats were exposed to 1g/kg toluene by oral gavage, and sacrificed after 4 hours. Total RNA was isolated from liver samples and gene expression analyzed using Affymetrix Rat 230 2.0 full-genome GeneChips. Data from 18 samples, with three rats in each of the control age groups and dosed age groups, were analyzed. Experiment Overall Design: F344 rats: Analysis of gene expression profiles for XMEs in male F344 6, 11, 18, and 24 months old. Total RNA was isolated from liver samples and gene expression analyzed using Affymetrix Rat 230 2.0 full-genome GeneChips. Data from 16 samples, with four rats in each of the 4 age groups, were analyzed.

2008-10-20 | E-GEOD-11097 | biostudies-arrayexpress

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