Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Insights into food intake response in anadromous Coilia nasus through stomach transcriptome analysis

ABSTRACT: Insights into food intake response in anadromous Coilia nasus through stomach transcriptome analysis

PROVIDER: PRJNA575877 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Insights into food intake response in anadromous Coilia nasus through stomach transcriptome analysis

Project description:To understand the molecular basis underlying the response to food intake, RNA sequencing was utilized to analyze the stomach transcriptome of C. nasus with feeding group (CSI) and non-feeding group (CSN).

2020-10-01 | GSE138446 | GEO

A proteomics approach reveals digestive and nutritional responses to food intake in anadromous Coilia nasus

Project description:The estuarine tapertail anchovy, Coilia nasus, is an anadromous fish that undertakes over a 600-km spawning migration along the Yangtze River of China. They generally cease feeding during this process, but we recently documented that a small proportion of them appear to feed. Research on proteomic responses is essential for understanding the phenomenon of C. nasus feeding. In this study, we used an iTRAQ-based proteomics approach to study the changes in protein expression in response to food intake in C. nasus following voluntary fasting. Coilia nasus in the feeding group (CSI) were fed shrimp or small fish, whereas those in the control group (CSN) were starved. We identified 3279 proteins in the gastric tissue/stomach, of which 279 were significantly differentially expressed. In all, 133 differentially expressed proteins (DEPs) were upregulated and 146 proteins were downregulated in CSI compared with those in CSN C. nasus. In addition to gastric acid secretion caused by gastric distention, a functional analysis suggested that a series of DEPs were involved mainly in the regulation of protein digestion (e.g., carboxypeptidase A1 and chymotrypsin A-like), immune response (e.g., lysozyme and alpha 2-macroglobulin), and nutrition metabolism (e.g., glyceraldehyde 3-phosphate dehydrogenase, glycogenin, long-chain acyl-CoA synthetase, and creatine kinase). Real-time PCR confirmed that the mRNA levels of the DEPs were similar those obtained using iTRAQ. These results indicate that the nutrients obtained through food were effectively utilized by C. nasus, thereby providing energy for swimming, gonadal maturation, primary metabolism, and an enhanced immune function to better resist pathogen interference. This research contributes to the elucidation of nutritional regulation mechanisms of C. nasus to better protect the wild population.

2022-05-25 | PXD033476 | Pride

Next Generation Sequencing Facilitates Quantitative Analysis of feeding and fasting coilia nasus liver Transcriptomes

Project description:Purpose: To gain a comprehensive understanding of differential gene expression in anadromous C. nasus during feeding and fasting conditions. Methods: we characterized transcriptomics of C. nasus livers between feeding and fasting using RNA-seq Results:A total of 23,159 mRNA moleculeswere selected to identify the mechanisms of feeding and fasting. Our results provide insight into the activation of protein synthesis and energy metabolism, identification of key genes involved in food intake regulation, motivation of the fatty acid biosynthesis, glycolysis, TCA cycle and oxidative phosphorylation and decrease of amino acids and linoleic acid metabolism in feeding conditions compared to fasting. Conclusions:This is the first study describing differential gene expression and metabolic changes accompanying feeding and fasting in this interesting but endangered group. Our findings will be useful for future research on feeding characteristics, energy utilization and survival strategies of C. nasus.

2020-05-01 | GSE142614 | GEO

Coilia nasus breed:Coilia nasus

Project description:Coilia nasus Genome sequencing

| PRJNA471747 | ENA

Coilia nasus

Project description:Coilia nasus Genome sequencing

| PRJNA272889 | ENA

Coilia nasus

Project description:Species delimitation in Coilia nasus

| PRJNA272838 | ENA

Coilia nasus

Project description:Coilia nasus Raw sequence reads